Homo sapiens

Lamp5 gabaergic interneuron

Middle temporal gyrus

43-year-old stage 68-year-old stage 88-year-old stage 86-year-old stage 82-year-old stage 83-year-old stage 60-year-old stage 75-year-old stage 80-year-old stage 77-year-old stage Adult stage 89-year-old stage 78-year-old stage 72-year-old stage 80 year-old and over stage 81-year-old stage 87-year-old stage 84-year-old stage 65-year-old stage 85-year-old stage 50-year-old stage 29-year-old stage 69-year-old stage 70-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Retinal cone cell Retinal rod cell

Fovea centralis Macula lutea proper Peripheral region of retina

83-year-old stage 78-year-old stage 84-year-old stage 65-year-old stage 73-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Subcutaneous adipocyte Mesenchymal stem cell of adipose tissue

Subcutaneous adipose tissue

34-year-old stage 24-year-old stage 33-year-old stage 41-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Renal medulla

65-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell B cell Neutrophil Mature nk t cell Smooth muscle cell Cd4-positive, alpha-beta t cell Macrophage Cd8-positive, alpha-beta t cell Erythrocyte Basal cell of prostate epithelium Luminal cell of prostate epithelium Endothelial cell Fibroblast Mast cell

Prostate gland

59-year-old stage 56-year-old stage 60-year-old stage

Normal

Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Colon goblet cell Tuft cell of colon Secretory cell Malignant cell Colonocyte Enteroendocrine cell of colon Intestinal crypt stem cell of colon Best4+ colonocyte Early colonocyte

Caecum Descending colon Sigmoid colon Hepatic flexure of colon Transverse colon Rectum

62-year-old stage 49-year-old stage 64-year-old stage 68-year-old stage 55-year-old stage 60-year-old stage 75-year-old stage 35-year-old stage 74-year-old stage 39-year-old stage 78-year-old stage 52-year-old stage 44-year-old stage 65-year-old stage 50-year-old stage 45-year-old stage 53-year-old stage 37-year-old stage 73-year-old stage 70-year-old stage 40-year-old stage 41-year-old stage

Colorectal cancer Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Regulatory t cell B cell Malignant cell Cd8-positive, alpha-beta t cell T follicular helper cell Exhausted t cell Plasma cell Myeloid cell Cd4-positive helper t cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Drosophila melanogaster

Sensory neuron of campaniform sensillum Embryonic/larval crystal cell Fat cell Chordotonal neuron Hemocyte Oenocyte Indirect flight muscle cell Mechanosensory neuron Tendon cell Glial cell

Body wall

Day 5 of adulthood

Normal

Female Male

10x 3' v3

Homo sapiens

Oligodendrocyte Natural killer cell Astrocyte T cell Medial ganglionic eminence derived interneuron Pvalb chandelier gabaergic interneuron Microglial cell Oligodendrocyte precursor cell Neuron Ependymal cell Pericyte Committed oligodendrocyte precursor Monocyte Eccentric medium spiny neuron Cerebellar inhibitory gabaergic interneuron Cerebellar granule cell Intratelencephalic-projecting glutamatergic cortical neuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Endothelial cell Amygdala excitatory neuron Caudal ganglionic eminence derived interneuron Thalamic excitatory neuron Central nervous system macrophage Midbrain-derived inhibitory neuron Fibroblast Lamp5 lhx6 neuron

Medial dorsal nucleus of thalamus

42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

T cell Malignant cell Macrophage Blood vessel smooth muscle cell Blood vessel endothelial cell Fibroblast

Axilla

27-year-old stage

Breast cancer

Female

10x 3' v3

Nucleus

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Macula densa epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2