Homo sapiens

Inhibitory interneuron

Prefrontal cortex

62-year-old stage 66-year-old stage 68-year-old stage 57-year-old stage 61-year-old stage 89-year-old stage 72-year-old stage 67-year-old stage 80 year-old and over stage 81-year-old stage 79-year-old stage 87-year-old stage 73-year-old stage 71-year-old stage

Alzheimer disease Normal

Female Male

10x 3' v2 10x 3' v3

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Cortex of kidney

64-year-old stage

Normal

Female

Slide-seqv2

Homo sapiens

Oligodendrocyte Natural killer cell Astrocyte Bergmann glial cell T cell Microglial cell Oligodendrocyte precursor cell B cell Neuron Ependymal cell Pericyte Committed oligodendrocyte precursor Monocyte Cerebellar inhibitory gabaergic interneuron Cerebellar granule cell Intratelencephalic-projecting glutamatergic cortical neuron Vascular associated smooth muscle cell Endothelial cell Caudal ganglionic eminence derived interneuron Choroid plexus epithelial cell Central nervous system macrophage Midbrain-derived inhibitory neuron Fibroblast Lamp5 lhx6 neuron Unipolar brush cell

Nucleus of medulla oblongata

42-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Dendritic cell Innate lymphoid cell B cell Neutrophil Macrophage Cd8-positive, alpha-beta t cell Plasma cell Mast cell Cd4-positive helper t cell

Gingiva Soft palate Hard palate Buccal mucosa

32-year-old stage 31-year-old stage 21-year-old stage 34-year-old stage 30-year-old stage Seventh decade stage 24-year-old stage 57-year-old stage 28-year-old stage 33-year-old stage 35-year-old stage 61-year-old stage 39-year-old stage 26-year-old stage Sixth decade stage 22-year-old stage 29-year-old stage 51-year-old stage

Normal

Female Male

10x 3' v2 10x 3' v3

Cell

Homo sapiens

Lamp5 gabaergic interneuron

Dorsolateral prefrontal cortex

42-year-old stage 68-year-old stage 88-year-old stage 86-year-old stage 82-year-old stage 83-year-old stage 75-year-old stage 80-year-old stage 77-year-old stage 89-year-old stage 78-year-old stage 72-year-old stage 80 year-old and over stage 81-year-old stage 87-year-old stage 84-year-old stage 85-year-old stage 50-year-old stage 29-year-old stage 69-year-old stage 70-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Renal medulla

72-year-old stage

Normal

Male

Slide-seqv2

Mus musculus

Astrocyte Obsolete excitatory neuron Oligodendrocyte precursor cell Brain microvascular endothelial cell Obsolete inhibitory neuron

Normal

10x 3' v2

Homo sapiens

Pericyte Endothelial cell of lymphatic vessel Smooth muscle cell Endothelial cell

Omental fat pad Subcutaneous adipose tissue

49-year-old stage 59-year-old stage 68-year-old stage 24-year-old stage 57-year-old stage 35-year-old stage 36-year-old stage 58-year-old stage 53-year-old stage 29-year-old stage 73-year-old stage 51-year-old stage 41-year-old stage 71-year-old stage

Normal

Female Male

Drop-seq 10x 3' v3

Nucleus Cell

Homo sapiens

Plasmacytoid dendritic cell Multiciliated columnar cell of tracheobronchial tree Natural killer cell Innate lymphoid cell T cell Connective tissue cell Serous cell of epithelium of trachea Regulatory t cell B cell Non-classical monocyte Tracheal goblet cell Neutrophil Monocyte Mature nk t cell Smooth muscle cell Cd4-positive, alpha-beta t cell Macrophage Myeloid dendritic cell Intermediate monocyte Cd8-positive, alpha-beta t cell Basal cell Endothelial cell Granulocyte Mucus secreting cell Ionocyte Fibroblast Plasma cell Mast cell

Trachea

59-year-old stage 56-year-old stage 60-year-old stage 61-year-old stage 67-year-old stage 45-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Plasmacytoid dendritic cell M cell of gut Intestine goblet cell Intestinal tuft cell Regulatory t cell B cell Endothelial cell of artery Glial cell Enterocyte Memory b cell Gamma-delta t cell Pericyte Iga plasma cell Conventional dendritic cell Endothelial cell of lymphatic vessel Monocyte Transit amplifying cell Cd4-positive, alpha-beta t cell Myofibroblast cell Enteroendocrine cell Cd8-positive, alpha-beta t cell T follicular helper cell Igg plasma cell Dendritic cell, human Vein endothelial cell Intestinal crypt stem cell Fibroblast Plasma cell Mast cell Myeloid cell Activated cd4-positive, alpha-beta t cell, human

Ileum

14-year-old stage 11-year-old stage 4-year-old stage 6-year-old stage 13-year-old stage 9-year-old stage 12-year-old stage 10-year-old stage

Crohn disease Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Kidney distal convoluted tubule epithelial cell

Renal medulla

56-year-old stage

Normal

Male

Slide-seqv2