Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

50-year-old stage

Normal

Male

Slide-seqv2

Mus musculus

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Midbrain-derived inhibitory neuron T cell Central nervous system macrophage Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Amygdala excitatory neuron Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Cerebellar inhibitory gabaergic interneuron Ependymal cell Microglial cell Thalamic excitatory neuron Choroid plexus epithelial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Astrocyte Fibroblast

Midbrain tectum

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Interneuron Progenitor cell Purkinje cell Gabaergic neuron

Medulla oblongata Hindbrain Telencephalon Midbrain Cerebellum Brain Diencephalon Pons

13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage 10th week post-fertilization stage Embryonic stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

Podocyte Endothelial cell Kidney interstitial fibroblast Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney granular cell Kidney collecting duct principal cell Kidney loop of henle thick ascending limb epithelial cell Macula densa epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

56-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Cerebellar granule cell Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Brodmann (1909) area 19

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Glutamatergic neuron Neuroblast (sensu nematoda and protostomia) Neural progenitor cell

Medulla oblongata Hindbrain Telencephalon Midbrain Cerebellum Brain Diencephalon Pons

13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage 10th week post-fertilization stage Embryonic stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

T cell Central nervous system macrophage Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte Committed oligodendrocyte precursor Lamp5 lhx6 neuron Pvalb chandelier gabaergic interneuron Medial ganglionic eminence derived interneuron Microglial cell B cell Pericyte Cerebral cortex neuron Near-projecting glutamatergic cortical neuron Neuron Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Caudal ganglionic eminence derived interneuron Vascular associated smooth muscle cell Sst chodl gabaergic interneuron Astrocyte Fibroblast

Inferior temporal gyrus

42-year-old stage 29-year-old stage 50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Club cell Airway submucosal gland duct basal cell Ciliated cell Lung secretory cell Neuroendocrine cell Basal cell Epithelial cell Myoepithelial cell Goblet cell Deuterosomal cell Serous secreting cell of bronchus submucosal gland Respiratory tract suprabasal cell

Bronchus Lung Trachea Upper lobe of left lung Lower lobe of left lung

66-year-old stage 75-year-old stage Sixth decade stage 29-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 64-year-old stage

Normal

Female Male

10x 5' transcription profiling 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

Intestine goblet cell Best4+ enterocyte Epithelial cell Enteroendocrine cell Intestinal crypt stem cell Enterocyte Progenitor cell

Duodenum Small intestine Ileum Jejunum

Carnegie stage 19 13th week post-fertilization stage 12th week post-fertilization stage 9th week post-fertilization stage 10th week post-fertilization stage Embryonic stage Carnegie stage 17 Carnegie stage 22 Carnegie stage 16 Carnegie stage 18 11th week post-fertilization stage

Normal

Female Male

10x 5' v2 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Neutrophil Blood vessel endothelial cell Natural killer cell Endothelial cell of lymphatic vessel Mast cell Cd8-positive, alpha-beta t cell Cd4-positive helper t cell Schwann cell Melanocyte Myoepithelial cell Macrophage Mural cell Keratinocyte Acinar cell of salivary gland Plasma cell Salivary gland glandular cell Langerhans cell Fibroblast Dendritic cell

Mucosa of lip Buccal mucosa

65-year-old stage 61-year-old stage 39-year-old stage 56-year-old stage 28-year-old stage 24-year-old stage 33-year-old stage 22-year-old stage

Normal

Female Male

10x 3' v3

Cell