Drosophila melanogaster

Muscle cell Adult tracheocyte Female germline cell Tracheolar cell Somatic precursor cell Epithelial cell Neuron Sensory neuron Eye photoreceptor cell Fat cell Adult neuron Hemocyte Male germline cell Oenocyte Neuron Glial cell

Trachea

Day 5 of adulthood

Normal

Female Male

10x 3' v3

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

50-year-old stage

Normal

Male

Slide-seqv2

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Ependymal cell Pericyte Endothelial cell Neuron Central nervous system macrophage Choroid plexus epithelial cell

Midbrain tectum

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Gabaergic neuron Interneuron Progenitor cell Purkinje cell

Diencephalon Cerebellum Telencephalon Brain Pons Midbrain Medulla oblongata Hindbrain

9th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 10th week post-fertilization stage Embryonic stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

56-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Brodmann (1909) area 19

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Glutamatergic neuron Neuroblast (sensu nematoda and protostomia) Neural progenitor cell

Diencephalon Cerebellum Telencephalon Brain Pons Midbrain Medulla oblongata Hindbrain

9th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 10th week post-fertilization stage Embryonic stage

Normal

Female Male

10x multiome

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Inferior temporal gyrus

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Myoepithelial cell Serous secreting cell of bronchus submucosal gland Club cell Basal cell Epithelial cell Deuterosomal cell Airway submucosal gland duct basal cell Neuroendocrine cell Lung secretory cell Ciliated cell Goblet cell Respiratory tract suprabasal cell

Lung Bronchus Upper lobe of left lung Trachea Lower lobe of left lung

66-year-old stage 64-year-old stage 75-year-old stage 59-year-old stage 58-year-old stage 35-year-old stage Seventh decade stage 29-year-old stage Sixth decade stage

Normal

Female Male

10x 3' v3 10x 5' transcription profiling 10x 3' v2

Cell Nucleus

Homo sapiens

Best4+ enterocyte Intestinal crypt stem cell Epithelial cell Enteroendocrine cell Progenitor cell Enterocyte Intestine goblet cell

Ileum Small intestine Jejunum Duodenum

9th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Embryonic stage 11th week post-fertilization stage Carnegie stage 19 Carnegie stage 17 Carnegie stage 18 Carnegie stage 16

Normal

Female Male

10x 5' v2 10x 3' v3 10x 3' v2

Cell