Homo sapiens

T cell Natural killer cell Pro-b cell Erythroid lineage cell Plasmacytoid dendritic cell Promonocyte Hematopoietic stem cell Common myeloid progenitor Plasma cell B cell Granulocyte monocyte progenitor cell Megakaryocyte-erythroid progenitor cell Monocyte Conventional dendritic cell

Bone marrow

66-year-old stage 75-year-old stage 21-year-old stage 13-year-old stage 65-year-old stage 42-year-old stage 26-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 2-year-old stage 52-year-old stage 54-year-old stage 48-year-old stage 30-year-old stage 39-year-old stage 44-year-old stage 17-year-old stage 29-year-old stage 38-year-old stage 56-year-old stage 8-year-old stage 55-year-old stage 84-year-old stage 15-year-old stage 50-year-old stage 80-year-old stage 28-year-old stage 51-year-old stage 57-year-old stage 12-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 9-year-old stage 14-year-old stage 24-year-old stage 16-year-old stage 23-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage Adult stage 67-year-old stage 2-month-old stage 3-year-old stage 32-year-old stage 77-year-old stage 33-year-old stage 8-month-old stage 18-month-old stage 64-year-old stage 45-year-old stage 62-year-old stage 19-month-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 31-year-old stage 36-year-old stage 1-year-old stage 22-year-old stage 19-year-old stage 63-year-old stage 20-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 5-year-old stage 11-year-old stage 6-year-old stage 7-year-old stage 10-year-old stage 4-year-old stage

Myeloid leukemia Normal

Female Male

10x transcription profiling 10x 3' v1 10x 5' v2 Sort-seq 10x 5' v1 Smart-seq2 Seq-well 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Central memory cd4-positive, alpha-beta t cell T cell Effector memory cd4-positive, alpha-beta t cell Memory regulatory t cell Naive regulatory t cell Memory ccr4-positive regulatory t cell Cd4-positive, alpha-beta memory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Cd8-positive, alpha-beta regulatory t cell Double negative thymocyte

Blood

34-year-old stage 65-year-old stage 26-year-old stage 61-year-old stage 30-year-old stage 29-year-old stage 56-year-old stage 55-year-old stage 28-year-old stage 57-year-old stage 12-year-old stage 35-year-old stage 58-year-old stage 59-year-old stage 25-year-old stage 32-year-old stage 33-year-old stage 64-year-old stage 62-year-old stage 60-year-old stage 31-year-old stage 36-year-old stage 63-year-old stage 11-year-old stage 27-year-old stage

Cytomegalovirus infection Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Mature gamma-delta t cell Cd8aa(i) thymocyte Cd8-positive, alpha-beta memory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Mucosal invariant t cell Central memory cd8-positive, alpha-beta t cell Double negative thymocyte Effector memory cd8-positive, alpha-beta t cell

Blood

66-year-old stage 69-year-old stage 75-year-old stage 65-year-old stage 42-year-old stage 89-year-old stage 43-year-old stage 87-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 44-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 80 year-old and over stage 49-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 77-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage 40-year-old stage

Cytomegalovirus infection Normal

Female Male

10x gene expression flex

Cell

Homo sapiens

Endothelial cell Mast cell Lymph node lymphatic vessel endothelial cell Smooth muscle cell Epithelial cell Lymphocyte Pulmonary alveolar type 1 cell Apoptosis fated cell Myeloid cell B cell Fibroblast Pulmonary alveolar type 2 cell

Chronic obstructive pulmonary disease Normal Pulmonary fibrosis Pulmonary systemic sclerosis

10x 3' v1 10x 3' v3 10x 3' v2

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Gamma-delta t cell Activated cd4-positive, alpha-beta t cell Mature nk t cell Mast cell Mammary gland epithelial cell Iga plasma cell Inflammatory macrophage Cd4-positive helper t cell Basal cell Effector memory cd4-positive, alpha-beta t cell Luminal epithelial cell of mammary gland Endothelial cell of artery Capillary endothelial cell Classical monocyte Plasmacytoid dendritic cell Alternatively activated macrophage Igg plasma cell Myeloid dendritic cell Lymphocyte Cd8-positive, alpha-beta memory t cell Macrophage Unswitched memory b cell Regulatory t cell Activated cd8-positive, alpha-beta t cell Myeloid cell Cd4-positive, alpha-beta t cell Vein endothelial cell Pericyte Vascular associated smooth muscle cell Naive b cell Conventional dendritic cell Fibroblast Class switched memory b cell Effector memory cd8-positive, alpha-beta t cell Non-classical monocyte

Breast

Adult stage Prime adult stage

Normal

Female

10x 3' v3 10x 3' v2

Cell

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Gamma-delta t cell Mast cell Cd8-positive, alpha-beta t cell Iga plasma cell Endothelial cell of artery Capillary endothelial cell Schwann cell Melanocyte Smooth muscle cell Sweat secreting cell Hair follicular keratinocyte Igg plasma cell Macrophage Innate lymphoid cell Regulatory t cell Skin fibroblast Plasmablast Keratinocyte Sebaceous gland cell Vein endothelial cell Pericyte Naive b cell Dendritic cell

Normal Atopic eczema

10x 3' v3

Homo sapiens

Oligodendrocyte precursor cell Oligodendrocyte Neural cell Microglial cell Astrocyte

Insular cortex Cingulate cortex Temporal cortex Caudal ganglionic eminence Frontal cortex Ganglionic eminence Cerebral cortex Lateral ganglionic eminence Primary motor cortex Medial ganglionic eminence

21-year-old stage 13-year-old stage 34-year-old stage Fifth lmp month stage Eighth lmp month stage 53-year-old stage 2-year-old stage 54-year-old stage 3-month-old stage 39-year-old stage 44-year-old stage 17-year-old stage 8-year-old stage 15-year-old stage 28-year-old stage 12-year-old stage Seventh lmp month stage Sixth lmp month stage 14-year-old stage 16-year-old stage 25-year-old stage Ninth lmp month stage 2-month-old stage 3-year-old stage Infant stage 45-year-old stage 1-month-old stage 1-year-old stage 22-year-old stage 19-year-old stage Newborn stage (0-28 days) 20-year-old stage 5-month-old stage 4-month-old stage 5-year-old stage 6-year-old stage 40-year-old stage 10-year-old stage Fourth lmp month stage 4-year-old stage

Normal

Female Male

10x multiome 10x 3' v3 10x 3' v2

Nucleus

Homo sapiens

Endothelial cell of lymphatic vessel Endothelial cell Mast cell Neural cell Lymphocyte Adipocyte Mural cell Myeloid cell Mesothelial cell Regular atrial cardiac myocyte Regular ventricular cardiac myocyte Fibroblast

Heart right ventricle Right cardiac atrium Apex of heart Sinoatrial node Heart left ventricle Left cardiac atrium Atrioventricular node Interventricular septum

Sixth decade stage Fifth decade stage Third decade stage Seventh decade stage Eighth decade stage

Normal

Female Male

10x multiome 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

T cell L2/3 intratelencephalic projecting glutamatergic neuron L5/6 near-projecting glutamatergic neuron Natural killer cell Oligodendrocyte precursor cell Endothelial cell Oligodendrocyte L6b glutamatergic neuron of the primary motor cortex Vascular leptomeningeal cell Smooth muscle cell Lamp5 gabaergic interneuron Microglial cell Vip gabaergic interneuron L2/3-6 intratelencephalic projecting glutamatergic neuron Plasma cell L5 extratelencephalic projecting glutamatergic cortical neuron L6 corticothalamic-projecting glutamatergic cortical neuron Pvalb gabaergic interneuron B cell Pericyte Perivascular macrophage L6 intratelencephalic projecting glutamatergic neuron Astrocyte Sst gabaergic interneuron Gabaergic neuron

Dorsolateral prefrontal cortex

66-year-old stage 69-year-old stage 75-year-old stage 65-year-old stage 89-year-old stage 87-year-old stage 71-year-old stage 85-year-old stage 88-year-old stage 54-year-old stage 68-year-old stage 84-year-old stage 82-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 80 year-old and over stage 67-year-old stage 77-year-old stage 64-year-old stage 86-year-old stage 70-year-old stage 78-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage

Amyotrophic lateral sclerosis Parkinson disease Diabetes mellitus Vascular dementia Frontotemporal dementia Normal Tauopathy Alzheimer disease Lewy body dementia Dementia Head injury

Female Male

10x 3' v3

Nucleus

Homo sapiens

Diffuse bipolar 1 cell Diffuse bipolar 4 cell Diffuse bipolar 2 cell Offx cell Rod bipolar cell Invaginating midget bipolar cell Retinal bipolar neuron Flat midget bipolar cell Diffuse bipolar 3a cell On-blue cone bipolar cell Diffuse bipolar 6 cell Giant bipolar cell Diffuse bipolar 3b cell

Normal

10x 3' v3

Homo sapiens

Diffuse bipolar 1 cell Diffuse bipolar 4 cell Diffuse bipolar 2 cell Offx cell Rod bipolar cell Invaginating midget bipolar cell Retinal bipolar neuron Flat midget bipolar cell Diffuse bipolar 3a cell On-blue cone bipolar cell Diffuse bipolar 6 cell Giant bipolar cell Diffuse bipolar 3b cell

Peripheral region of retina Macula lutea proper Fovea centralis Macula lutea

66-year-old stage 69-year-old stage 75-year-old stage 21-year-old stage 34-year-old stage 65-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 88-year-old stage 52-year-old stage 68-year-old stage 30-year-old stage 17-year-old stage 84-year-old stage 15-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 80 year-old and over stage 24-year-old stage 16-year-old stage 58-year-old stage 25-year-old stage 67-year-old stage Late adult stage 90 year-old and over stage 3-year-old stage 32-year-old stage 77-year-old stage 64-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 18-year-old stage 60-79 year-old stage 63-year-old stage Eighth decade stage 47-year-old stage 76-year-old stage 73-year-old stage 83-year-old stage Prime adult stage 11-year-old stage 27-year-old stage 10-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Central memory cd4-positive, alpha-beta t cell Cd1c-positive myeloid dendritic cell Natural killer cell Gamma-delta t cell Hematopoietic precursor cell Platelet Cd141-positive myeloid dendritic cell Effector memory cd4-positive, alpha-beta t cell Plasmacytoid dendritic cell Naive thymus-derived cd8-positive, alpha-beta t cell Memory b cell Innate lymphoid cell Regulatory t cell Plasmablast Cd4-positive, alpha-beta t cell Cd14-positive monocyte Cd14-low, cd16-positive monocyte B cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Central memory cd8-positive, alpha-beta t cell Naive b cell Double negative thymocyte Cd16-negative, cd56-bright natural killer cell, human Effector memory cd8-positive, alpha-beta t cell

Blood

20-year-old stage

Normal

Male

Scalebio single cell rna sequencing

Cell