Homo sapiens

Pro-b cell Common myeloid progenitor Promonocyte Granulocyte monocyte progenitor cell Megakaryocyte-erythroid progenitor cell Erythroid lineage cell Monocyte Conventional dendritic cell Hematopoietic stem cell B cell Plasma cell T cell Plasmacytoid dendritic cell Natural killer cell

Bone marrow

80-year-old stage 85-year-old stage 66-year-old stage 19-year-old stage 16-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 57-year-old stage 76-year-old stage 19-month-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 22-year-old stage 44-year-old stage 48-year-old stage 64-year-old stage 45-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 67-year-old stage 1-year-old stage 2-year-old stage 75-year-old stage 8-month-old stage 23-year-old stage 71-year-old stage Adult stage 14-year-old stage 78-year-old stage 6-year-old stage 28-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 20-year-old stage 47-year-old stage 31-year-old stage 2-month-old stage 56-year-old stage 58-year-old stage 61-year-old stage 15-year-old stage 8-year-old stage 51-year-old stage 42-year-old stage 35-year-old stage 53-year-old stage 10-year-old stage 17-year-old stage 72-year-old stage 73-year-old stage 30-year-old stage 24-year-old stage 13-year-old stage 3-year-old stage 26-year-old stage 84-year-old stage 12-year-old stage 7-year-old stage 39-year-old stage 4-year-old stage 18-month-old stage 9-year-old stage 43-year-old stage 5-year-old stage 29-year-old stage 38-year-old stage 41-year-old stage 81-year-old stage 55-year-old stage 11-year-old stage

Normal Myeloid leukemia

Female Male

Smart-seq2 10x 5' v2 10x 5' v1 10x 3' v3 10x transcription profiling Sort-seq 10x 3' v1 Seq-well 10x 3' v2

Cell

Homo sapiens

Cd4-positive, alpha-beta memory t cell Cd8-positive, alpha-beta regulatory t cell Naive regulatory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Effector memory cd4-positive, alpha-beta t cell Double negative thymocyte Central memory cd4-positive, alpha-beta t cell T cell Memory regulatory t cell Memory ccr4-positive regulatory t cell

Blood

32-year-old stage 57-year-old stage 62-year-old stage 60-year-old stage 64-year-old stage 25-year-old stage 33-year-old stage 65-year-old stage 27-year-old stage 28-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 31-year-old stage 56-year-old stage 58-year-old stage 61-year-old stage 34-year-old stage 35-year-old stage 30-year-old stage 26-year-old stage 12-year-old stage 29-year-old stage 55-year-old stage 11-year-old stage

Normal Cytomegalovirus infection

Female Male

10x 3' v3

Cell

Homo sapiens

Mature gamma-delta t cell Mucosal invariant t cell Double negative thymocyte Cd8-positive, alpha-beta memory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell Cd8aa(i) thymocyte

Blood

80-year-old stage 85-year-old stage 82-year-old stage 66-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 80 year-old and over stage 44-year-old stage 48-year-old stage 64-year-old stage 89-year-old stage 45-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage 40-year-old stage 75-year-old stage 71-year-old stage 78-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 47-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 53-year-old stage 72-year-old stage 73-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 87-year-old stage 41-year-old stage 81-year-old stage 55-year-old stage 83-year-old stage

Normal Cytomegalovirus infection

Female Male

10x gene expression flex

Cell

Homo sapiens

Pulmonary alveolar type 1 cell Pulmonary alveolar type 2 cell Lymphocyte Lymph node lymphatic vessel endothelial cell Mast cell Epithelial cell Fibroblast Endothelial cell B cell Smooth muscle cell Apoptosis fated cell Myeloid cell

Normal Chronic obstructive pulmonary disease Pulmonary fibrosis Pulmonary systemic sclerosis

10x 3' v3 10x 3' v1 10x 3' v2

Homo sapiens

Capillary endothelial cell Inflammatory macrophage Activated cd8-positive, alpha-beta t cell Naive b cell Igg plasma cell Classical monocyte Endothelial cell of lymphatic vessel Cd4-positive, alpha-beta t cell Class switched memory b cell Non-classical monocyte Iga plasma cell Lymphocyte Macrophage Unswitched memory b cell Effector memory cd4-positive, alpha-beta t cell Mast cell Alternatively activated macrophage Basal cell Cd4-positive helper t cell Neutrophil Myeloid dendritic cell Luminal epithelial cell of mammary gland Vein endothelial cell Cd8-positive, alpha-beta memory t cell Vascular associated smooth muscle cell Conventional dendritic cell Fibroblast Effector memory cd8-positive, alpha-beta t cell Pericyte Activated cd4-positive, alpha-beta t cell Regulatory t cell Mature nk t cell T cell Plasmacytoid dendritic cell Natural killer cell Endothelial cell of artery Gamma-delta t cell Mammary gland epithelial cell Myeloid cell

Breast

Prime adult stage Adult stage

Normal

Female

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Schwann cell Skin fibroblast Capillary endothelial cell Naive b cell Igg plasma cell Innate lymphoid cell Endothelial cell of lymphatic vessel Iga plasma cell Macrophage Mast cell Sebaceous gland cell Neutrophil Plasmablast Vein endothelial cell Pericyte Sweat secreting cell Cd8-positive, alpha-beta t cell Dendritic cell Regulatory t cell T cell Smooth muscle cell Hair follicular keratinocyte Keratinocyte Natural killer cell Endothelial cell of artery Gamma-delta t cell Melanocyte

Atopic eczema Normal

10x 3' v3

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Microglial cell Neural cell

Lateral ganglionic eminence Cingulate cortex Insular cortex Temporal cortex Medial ganglionic eminence Frontal cortex Cerebral cortex Primary motor cortex Ganglionic eminence Caudal ganglionic eminence

19-year-old stage 16-year-old stage 3-month-old stage 21-year-old stage Ninth lmp month stage 22-year-old stage 5-month-old stage 4-month-old stage 44-year-old stage 45-year-old stage Sixth lmp month stage 25-year-old stage 1-year-old stage 2-year-old stage 40-year-old stage 1-month-old stage 14-year-old stage Fifth lmp month stage 6-year-old stage 28-year-old stage Infant stage 54-year-old stage Seventh lmp month stage 20-year-old stage 2-month-old stage 15-year-old stage 34-year-old stage 8-year-old stage Eighth lmp month stage 53-year-old stage 10-year-old stage 17-year-old stage 13-year-old stage Fourth lmp month stage 3-year-old stage 12-year-old stage 39-year-old stage Newborn stage (0-28 days) 4-year-old stage 5-year-old stage

Normal

Female Male

10x 3' v3 10x multiome 10x 3' v2

Nucleus

Homo sapiens

Mural cell Endothelial cell of lymphatic vessel Lymphocyte Mesothelial cell Mast cell Adipocyte Regular atrial cardiac myocyte Fibroblast Endothelial cell Regular ventricular cardiac myocyte Myeloid cell Neural cell

Interventricular septum Apex of heart Heart left ventricle Sinoatrial node Left cardiac atrium Atrioventricular node Right cardiac atrium Heart right ventricle

Eighth decade stage Fifth decade stage Seventh decade stage Third decade stage Sixth decade stage

Normal

Female Male

10x 3' v3 10x multiome 10x 3' v2

Cell Nucleus

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron L2/3 intratelencephalic projecting glutamatergic neuron L6 intratelencephalic projecting glutamatergic neuron Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Gabaergic neuron L6b glutamatergic neuron of the primary motor cortex L6 corticothalamic-projecting glutamatergic cortical neuron Sst gabaergic interneuron L5/6 near-projecting glutamatergic neuron Microglial cell Lamp5 gabaergic interneuron Pericyte Endothelial cell B cell Plasma cell T cell Perivascular macrophage Smooth muscle cell L5 extratelencephalic projecting glutamatergic cortical neuron Natural killer cell Vip gabaergic interneuron Pvalb gabaergic interneuron

Dorsolateral prefrontal cortex

80-year-old stage 85-year-old stage 82-year-old stage 66-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 86-year-old stage 76-year-old stage 80 year-old and over stage 64-year-old stage 89-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage 75-year-old stage 71-year-old stage 78-year-old stage 54-year-old stage 68-year-old stage 72-year-old stage 73-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 87-year-old stage 81-year-old stage 83-year-old stage

Normal Frontotemporal dementia Lewy body dementia Tauopathy Vascular dementia Head injury Dementia Parkinson disease Amyotrophic lateral sclerosis Alzheimer disease Diabetes mellitus

Female Male

10x 3' v3

Nucleus

Homo sapiens

Diffuse bipolar 4 cell Flat midget bipolar cell Offx cell Diffuse bipolar 2 cell Diffuse bipolar 3a cell Diffuse bipolar 3b cell Invaginating midget bipolar cell Giant bipolar cell Diffuse bipolar 6 cell Diffuse bipolar 1 cell Retinal bipolar neuron On-blue cone bipolar cell Rod bipolar cell

Macula lutea Peripheral region of retina Fovea centralis Macula lutea proper

80-year-old stage 85-year-old stage 82-year-old stage 66-year-old stage 16-year-old stage 70-year-old stage 18-year-old stage 77-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 86-year-old stage 76-year-old stage 62-year-old stage Prime adult stage 46-year-old stage 52-year-old stage 60-year-old stage 60-79 year-old stage 80 year-old and over stage 64-year-old stage 25-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage Eighth decade stage 75-year-old stage 27-year-old stage 71-year-old stage 78-year-old stage 63-year-old stage 47-year-old stage 58-year-old stage 68-year-old stage 15-year-old stage 34-year-old stage 53-year-old stage 10-year-old stage 17-year-old stage 72-year-old stage 73-year-old stage 30-year-old stage 24-year-old stage 3-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 90 year-old and over stage Late adult stage 41-year-old stage 81-year-old stage 83-year-old stage 11-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Cd1c-positive myeloid dendritic cell Naive b cell Innate lymphoid cell Cd4-positive, alpha-beta t cell Platelet Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd4-positive, alpha-beta t cell Cd141-positive myeloid dendritic cell Cd16-negative, cd56-bright natural killer cell, human Cd14-positive monocyte Double negative thymocyte Plasmablast Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Hematopoietic precursor cell Central memory cd8-positive, alpha-beta t cell B cell Regulatory t cell Memory b cell Plasmacytoid dendritic cell Cd14-low, cd16-positive monocyte Natural killer cell Gamma-delta t cell

Blood

20-year-old stage

Normal

Male

Scalebio single cell rna sequencing

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Renal medulla Cortex of kidney

50-year-old stage 62-year-old stage 64-year-old stage 65-year-old stage 54-year-old stage 56-year-old stage 53-year-old stage 72-year-old stage

Normal

Female Male

Slide-seqv2