Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Ependymal cell Pericyte Endothelial cell Neuron Central nervous system macrophage

Bed nucleus of stria terminalis

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Brodmann (1909) area 13

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Club cell Ionocyte Glandular secretory epithelial cell Respiratory tract multiciliated cell Respiratory tract epithelial cell Nasal mucosa goblet cell Keratinizing barrier epithelial cell T cell Respiratory basal cell Keratinocyte Professional antigen presenting cell

Nasal cavity epithelium

66-year-old stage 64-year-old stage 65-year-old stage 63-year-old stage 59-year-old stage 34-year-old stage 30-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Cd8-positive, alpha-beta thymocyte Follicular b cell Innate lymphoid cell Cd4-positive, alpha-beta memory t cell Cd4-positive, alpha-beta t cell Intestinal crypt stem cell Macrophage Cd8-alpha-beta-positive, alpha-beta intraepithelial t cell Mast cell Enteroendocrine cell Fibroblast Transit amplifying cell of colon Intestinal tuft cell B cell Activated cd4-positive, alpha-beta t cell Dendritic cell Plasma cell Regulatory t cell T cell Enterocyte Natural killer cell M cell of gut Goblet cell

Caecum epithelium Colonic epithelium Mucosa of transverse colon

Adult stage

Normal

Female Male

10x 3' v1 10x 3' v2

Cell

Homo sapiens

Retinal rod cell Astrocyte Retinal cone cell Off-bipolar cell Mast cell Endothelial cell of vascular tree On-bipolar cell Monocyte Microglial cell Fibroblast Pericyte Mueller cell T cell Amacrine cell Retinal pigment epithelial cell Retinal ganglion cell Retina horizontal cell Natural killer cell Melanocyte Rod bipolar cell

Retina Pigment epithelium of eye

Adult stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Cell Macroglial cell Endothelial cell

Middle temporal gyrus Putamen Brodmann (1909) area 24 Posterior parahippocampal gyrus Brodmann (1909) area 38 Secondary visual cortex Brodmann (1909) area 19 Inferior temporal gyrus Hippocampal formation Dentate gyrus of hippocampal formation Bed nucleus of stria terminalis Primary somatosensory cortex Substantia innominata Body of caudate nucleus Cerebellum Insula Central nucleus of inferior colliculus Central amygdaloid nucleus Granular insular cortex Agranular insular cortex Globus pallidus Basolateral amygdaloid nuclear complex Anterior cingulate cortex Subiculum Primary auditory cortex Medial entorhinal cortex Corticomedial nuclear complex Brodmann (1909) area 46 Lateral entorhinal cortex Brodmann (1909) area 25 Parietal cortex Primary motor cortex Pulvinar nucleus Amygdala Primary visual cortex Hippocampal field Piriform cortex Claustrum of brain Pontine nuclear group Medial dorsal nucleus of thalamus Nucleus accumbens Septal nuclear complex Prefrontal cortex Cuneus cortex

58-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Cell Macroglial cell Endothelial cell

Middle temporal gyrus Putamen Brodmann (1909) area 24 Posterior parahippocampal gyrus Brodmann (1909) area 38 Secondary visual cortex Brodmann (1909) area 19 Inferior temporal gyrus Hippocampal formation Dentate gyrus of hippocampal formation Bed nucleus of stria terminalis Primary somatosensory cortex Substantia innominata Body of caudate nucleus Cerebellum Insula Central nucleus of inferior colliculus Central amygdaloid nucleus Granular insular cortex Agranular insular cortex Globus pallidus Basolateral amygdaloid nuclear complex Anterior cingulate cortex Subiculum Primary auditory cortex Medial entorhinal cortex Corticomedial nuclear complex Brodmann (1909) area 46 Lateral entorhinal cortex Brodmann (1909) area 25 Parietal cortex Primary motor cortex Pulvinar nucleus Amygdala Primary visual cortex Hippocampal field Piriform cortex Claustrum of brain Pontine nuclear group Medial dorsal nucleus of thalamus Nucleus accumbens Septal nuclear complex Prefrontal cortex Cuneus cortex

58-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

Snmc-seq2

Nucleus

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Myofibroblast cell Type i nk t cell Obsolete somatic cell Mesenchymal stem cell Classical monocyte Innate lymphoid cell Kidney connecting tubule epithelial cell Cd4-positive, alpha-beta t cell Kidney tubule cell Non-classical monocyte Macrophage Epithelial fate stem cell Stromal cell Mast cell Epithelial cell Erythroid lineage cell Neutrophil Monocyte Megakaryocyte Renal beta-intercalated cell Renal principal cell Kidney resident macrophage Epithelial cell of proximal tubule Conventional dendritic cell Fibroblast Renal intercalated cell Podocyte Tissue-resident macrophage Endothelial cell Mesenchymal cell Kidney loop of henle epithelial cell Kidney pelvis urothelial cell B cell Cd8-positive, alpha-beta t cell Dendritic cell Peritubular capillary endothelial cell Plasmacytoid dendritic cell Renal alpha-intercalated cell Glomerular endothelial cell Embryonic fibroblast Neuron Vasa recta cell Natural killer cell

Metanephros Adult mammalian kidney

13th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 17th week post-fertilization stage 53-year-old stage 14th week post-fertilization stage 72-year-old stage Carnegie stage 23

Normal

Female Male

10x genomics v2

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Macula densa epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Putamen

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney granular cell Leukocyte Macrophage Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Endothelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Kidney

6-month-old stage

Normal

Male

Slide-seqv2