Homo sapiens

Glutamatergic neuron

Brodmann (1909) area 9 Brodmann (1909) area 7 Brodmann (1909) area 17

66-year-old stage 75-year-old stage 91-year-old stage 89-year-old stage 93-year-old stage 87-year-old stage 71-year-old stage 85-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 68-year-old stage 92-year-old stage 111-year-old stage 82-year-old stage 80-year-old stage 57-year-old stage 81-year-old stage 97-year-old stage 58-year-old stage 67-year-old stage 62-year-old stage 86-year-old stage 70-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage

Normal Alzheimer disease

Female Male

10x 3' v3 Drop-seq 10x 3' v2

Nucleus

Homo sapiens

T cell Neutrophil Natural killer cell Endothelial cell of lymphatic vessel Gamma-delta t cell Mast cell Cd8-positive, alpha-beta t cell Iga plasma cell Endothelial cell of artery Capillary endothelial cell Schwann cell Melanocyte Smooth muscle cell Sweat secreting cell Hair follicular keratinocyte Igg plasma cell Macrophage Innate lymphoid cell Regulatory t cell Skin fibroblast Plasmablast Keratinocyte Sebaceous gland cell Vein endothelial cell Pericyte Naive b cell Dendritic cell

Normal Atopic eczema

10x 3' v3 10x 3' v2

Macaca mulatta

Oligodendrocyte

Dorsolateral prefrontal cortex Brodmann (1909) area 19 Medial dorsal nucleus of thalamus Primary visual cortex Ventral lateral nucleus of thalamus Inferior temporal gyrus Medulla oblongata Prefrontal cortex Midbrain Entorhinal cortex Nucleus accumbens Cerebrocerebellum Primary somatosensory cortex Primary auditory cortex Primary motor cortex Inferior parietal cortex Head of caudate nucleus Corpus callosum Hippocampal formation Perirhinal cortex Pons Cerebellar vermis Anterior cingulate cortex Superior temporal sulcus Superior parietal cortex Lateral geniculate body Amygdala

Juvenile stage Prime adult stage

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Oligodendrocyte

Cranial nerve ii Optic disc

69-year-old stage 37-year-old stage 75-year-old stage 21-year-old stage 34-year-old stage 65-year-old stage 42-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 30-year-old stage 17-year-old stage 56-year-old stage 15-year-old stage 82-year-old stage 50-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 16-year-old stage 58-year-old stage 67-year-old stage 90 year-old and over stage 3-year-old stage 32-year-old stage 77-year-old stage 33-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 18-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 11-year-old stage 27-year-old stage 10-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Mus musculus

Glutamatergic neuron Endothelial cell Oligodendrocyte Smooth muscle cell Microglial cell Leptomeningeal cell Pericyte Astrocyte Gabaergic neuron

Primary motor cortex

Prime adult stage

Normal

Male

Merfish

Homo sapiens

Cycling t cell Natural killer cell Mast cell Exhausted t cell Cd4-positive helper t cell Effector memory cd4-positive, alpha-beta t cell Plasmacytoid dendritic cell Igg plasma cell Myeloid dendritic cell Cycling macrophage Macrophage Cd4-positive, cd25-positive, ccr4-positive, alpha-beta regulatory t cell Memory b cell T follicular helper cell Naive t cell Cd8-positive, alpha-beta regulatory t cell Conventional dendritic cell Effector memory cd8-positive, alpha-beta t cell

Breast

66-year-old stage 69-year-old stage 37-year-old stage Middle aged stage 34-year-old stage 65-year-old stage 42-year-old stage 43-year-old stage Sixth decade stage 71-year-old stage 85-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 30-year-old stage 29-year-old stage 38-year-old stage Fifth decade stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 57-year-old stage 80 year-old and over stage 49-year-old stage 35-year-old stage Seventh decade stage 58-year-old stage 59-year-old stage 25-year-old stage Adult stage 67-year-old stage Late adult stage 32-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 60-79 year-old stage 36-year-old stage 63-year-old stage Eighth decade stage 47-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage Prime adult stage Ninth decade stage

Invasive ductal breast carcinoma Breast cancer Estrogen-receptor positive breast cancer Breast apocrine carcinoma Invasive lobular breast carcinoma Metaplastic breast carcinoma Her2 positive breast carcinoma Breast mucinous carcinoma Triple-negative breast carcinoma Invasive tubular breast carcinoma Breast carcinoma

Female Male

10x 5' v2 10x 5' transcription profiling 10x 5' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Retinal astrocyte Astrocyte

Cranial nerve ii Optic disc

69-year-old stage 37-year-old stage 75-year-old stage 21-year-old stage 34-year-old stage 65-year-old stage 42-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 30-year-old stage 17-year-old stage 56-year-old stage 15-year-old stage 82-year-old stage 50-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 16-year-old stage 58-year-old stage 25-year-old stage 67-year-old stage 90 year-old and over stage 3-year-old stage 32-year-old stage 77-year-old stage 33-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 18-year-old stage Newborn stage (0-28 days) 63-year-old stage 47-year-old stage 76-year-old stage 11-year-old stage 27-year-old stage 10-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Germinal center b cell Precursor b cell Memory b cell Plasmablast Plasma cell B cell Naive b cell

Lung Inguinal lymph node Jejunal epithelium Epithelial lining fluid Thoracic lymph node Spleen Jejunum lamina propria Blood Bone marrow Mesenteric lymph node

75-year-old stage Sixth decade stage 68-year-old stage 48-year-old stage 56-year-old stage Fifth decade stage Third decade stage 23-year-old stage 25-year-old stage 67-year-old stage 33-year-old stage 64-year-old stage Fourth decade stage 20-year-old stage Eighth decade stage 73-year-old stage 27-year-old stage

Normal

Female Male

10x 5' v2 10x 3' v3

Cell

Homo sapiens

Tracheobronchial smooth muscle cell Lung megakaryocyte Mature conventional dendritic cell Ciliated cell Natural killer cell Lung secretory cell Secretory cell Mast cell Lung interstitial macrophage Alveolar capillary type 1 endothelial cell Pulmonary neuroendocrine cell Serous secreting cell Basal cell Endothelial cell of respiratory system lymphatic vessel Endothelial cell of artery Classical monocyte Plasmacytoid dendritic cell Cd8-positive, alpha-beta cytotoxic t cell Goblet cell Brush cell of tracheobronchial tree Mesothelial cell of pleura Mucus secreting cell of tracheobronchial tree submucosal gland Regulatory t cell Pulmonary alveolar type 1 cell Helper t cell Alveolar macrophage Plasma cell Vein endothelial cell Secondary crest myofibroblast Alveolar adventitial fibroblast Vein endothelial cell of respiratory system B cell Alveolar capillary type 2 endothelial cell Pericyte Alveolar type 1 fibroblast cell Vascular associated smooth muscle cell Conventional dendritic cell Pulmonary alveolar type 2 cell Non-classical monocyte

Middle lobe of right lung

15-month-old stage 6-month-old stage 21-month-old stage 13-month-old stage 20-month-old stage 10-month-old stage 3-year-old stage 8-month-old stage 18-month-old stage 19-month-old stage 5-month-old stage 4-month-old stage 11-month-old stage 12-month-old stage 7-month-old stage 14-month-old stage

Normal Bronchopulmonary dysplasia

Female Male

10x 3' v3

Nucleus

Homo sapiens

Podocyte Natural killer cell Endothelial cell of lymphatic vessel Renal beta-intercalated cell Gamma-delta t cell Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Iga plasma cell Effector memory cd4-positive, alpha-beta t cell Activated type ii nk t cell Classical monocyte Epithelial cell of proximal tubule Epithelial cell Plasmacytoid dendritic cell Igg plasma cell Epithelial cell of proximal tubule segment 1 Renal alpha-intercalated cell Naive thymus-derived cd8-positive, alpha-beta t cell Macrophage Unswitched memory b cell Cd4-positive, cd25-positive, alpha-beta regulatory t cell Kidney distal convoluted tubule epithelial cell Kidney pelvis urothelial cell Kidney collecting duct principal cell Innate lymphoid cell Kidney connecting tubule principal cell Kidney loop of henle thick ascending limb epithelial cell Mhc-ii-positive classical monocyte Epithelial cell of proximal tubule segment 3 Plasma cell Cd4-positive, alpha-beta t cell B cell Pericyte Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Collagen secreting cell Naive b cell Kidney loop of henle ascending limb epithelial cell Conventional dendritic cell Fibroblast Class switched memory b cell Effector memory cd8-positive, alpha-beta t cell Non-classical monocyte

Perirenal fat Vein Kidney Adrenal tissue Blood

Sixth decade stage Fifth decade stage Seventh decade stage Eighth decade stage

Kidney benign neoplasm Normal Nonpapillary renal cell carcinoma Kidney oncocytoma

Female Male

10x 5' transcription profiling

Cell

Homo sapiens

Gabaergic amacrine cell Retinal ganglion cell Retinal pigment epithelial cell Rod bipolar cell S cone cell Retinal bipolar neuron H2 horizontal cell Microglial cell Parasol ganglion cell of retina Retinal cone cell Glycinergic amacrine cell Retinal rod cell Amacrine cell Starburst amacrine cell Midget ganglion cell of retina Mueller cell Astrocyte H1 horizontal cell

Peripheral region of retina Macula lutea proper Fovea centralis Chorioretinal region Retinal neural layer

69-year-old stage 65-year-old stage 42-year-old stage 89-year-old stage 71-year-old stage 53-year-old stage 54-year-old stage 68-year-old stage 82-year-old stage 80-year-old stage 74-year-old stage Adult stage 64-year-old stage 86-year-old stage 60-year-old stage 78-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Central memory cd4-positive, alpha-beta t cell Germinal center b cell Stromal cell Natural killer cell Gastrointestinal tract (lamina propria) macrophage of large intestine Endothelial cell of lymphatic vessel Gamma-delta t cell Best4+ enterocyte Mast cell Stem cell Effector memory cd4-positive, alpha-beta t cell Endothelial cell of artery Capillary endothelial cell Paneth cell Enteroendocrine cell Goblet cell T-helper 17 cell Macrophage Tuft cell of colon Memory b cell Follicular b cell Innate lymphoid cell Regulatory t cell Glial cell Enterocyte Plasma cell Vein endothelial cell Transit amplifying cell T follicular helper cell B cell Pericyte T-helper 1 cell Monocyte Naive b cell Enterocyte of epithelium of large intestine Myofibroblast cell Conventional dendritic cell Effector memory cd8-positive, alpha-beta t cell

Ascending colon Duodenum Colonic epithelium Caecum Small intestine Ileum Vermiform appendix Rectum Jejunum Large intestine Transverse colon Descending colon Sigmoid colon

Sixth decade stage 53-year-old stage 29-year-old stage Fifth decade stage Third decade stage Seventh decade stage Adult stage 45-year-old stage Eighth decade stage

Normal

Female Male

10x 3' v1 10x 5' v2 10x 3' v3 10x 3' v2

Cell