Mus musculus

Inhibitory interneuron Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Macrophage Medium spiny neuron Cell Microglial cell Pericyte Endothelial cell T cell Neuron

Brain ventricle Corpus callosum Cortical layer ii/iii Cortical layer vi Pia mater Olfactory region Striatum Cortical layer v

20-month-old stage and over 4-week-old stage 24-week-old stage

Normal

Female

Merfish

Homo sapiens

Off parasol ganglion cell On midget ganglion cell On parasol ganglion cell Retinal ganglion cell Off midget ganglion cell

Macula lutea Peripheral region of retina Fovea centralis Macula lutea proper

80-year-old stage 85-year-old stage 82-year-old stage 66-year-old stage 16-year-old stage 70-year-old stage 18-year-old stage 77-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 86-year-old stage 76-year-old stage 62-year-old stage Prime adult stage 46-year-old stage 52-year-old stage 60-year-old stage 60-79 year-old stage 80 year-old and over stage 64-year-old stage 25-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage Eighth decade stage 75-year-old stage 27-year-old stage 71-year-old stage 78-year-old stage 63-year-old stage 47-year-old stage 58-year-old stage 68-year-old stage 15-year-old stage 34-year-old stage 53-year-old stage 10-year-old stage 17-year-old stage 72-year-old stage 73-year-old stage 30-year-old stage 3-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 90 year-old and over stage Late adult stage 41-year-old stage 81-year-old stage 83-year-old stage 11-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron

Dorsolateral prefrontal cortex

80-year-old stage 85-year-old stage 82-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 80 year-old and over stage 89-year-old stage 75-year-old stage 78-year-old stage 68-year-old stage 42-year-old stage 72-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 29-year-old stage 87-year-old stage 81-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x 3' v3 10x multiome

Nucleus

Homo sapiens

Radial glial cell Mural cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Macrophage Mast cell Monocyte Microglial cell Malignant cell Endothelial cell B cell Dendritic cell Plasma cell Neuron Natural killer cell Mature t cell

Right temporal lobe Forebrain Left temporal lobe Right parietal lobe Temporoparietal junction Right frontal lobe Brain Left frontal lobe Temporal lobe Frontal lobe Left parietal lobe

80-year-old stage 66-year-old stage 70-year-old stage 77-year-old stage 21-year-old stage 50-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 44-year-old stage 48-year-old stage 64-year-old stage 45-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 67-year-old stage 71-year-old stage Adult stage 78-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 34-year-old stage 51-year-old stage 42-year-old stage 53-year-old stage 72-year-old stage 69-year-old stage 55-year-old stage 83-year-old stage

Glioblastoma

Female Male

Smart-seq2 Cel-seq2 10x 5' v1 10x 3' v3 Bd rhapsody whole transcriptome analysis 10x 3' v2 Strt-seq Microwell-seq

Cell

Homo sapiens

Naive b cell Classical monocyte Class switched memory b cell Non-classical monocyte Leukocyte Mucosal invariant t cell Unswitched memory b cell Effector memory cd4-positive, alpha-beta t cell Effector memory cd4-positive, alpha-beta t cell, terminally differentiated Cd16-negative, cd56-bright natural killer cell, human Megakaryocyte Plasmablast Conventional dendritic cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell Plasma cell Regulatory t cell Memory b cell Plasmacytoid dendritic cell Effector memory cd8-positive, alpha-beta t cell, terminally differentiated Hematopoietic multipotent progenitor cell Gamma-delta t cell Cd16-positive, cd56-dim natural killer cell, human

Blood

70-year-old stage 21-year-old stage 32-year-old stage 57-year-old stage 62-year-old stage 46-year-old stage 22-year-old stage 44-year-old stage 64-year-old stage 45-year-old stage 33-year-old stage 40-year-old stage Eighth decade stage 27-year-old stage 37-year-old stage 71-year-old stage Adult stage 28-year-old stage 63-year-old stage 59-year-old stage 58-year-old stage 34-year-old stage 51-year-old stage 49-year-old stage 35-year-old stage 53-year-old stage 73-year-old stage 30-year-old stage Seventh decade stage 26-year-old stage 39-year-old stage 69-year-old stage Late adult stage 29-year-old stage 38-year-old stage 55-year-old stage Sixth decade stage

Normal

Female Male

10x 5' v2 10x 5' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Macrophage Megakaryocyte-erythroid progenitor cell Erythroid lineage cell Neutrophil Megakaryocyte Fibroblast Malignant cell B cell Plasma cell T cell Natural killer cell Myeloid cell

Pleural fluid Blood Bone marrow

Nursing stage (0-11 months) Pediatric stage

T-cell childhood acute lymphocytic leukemia

Female Male

10x 5' v2

Cell

Homo sapiens

Schwann cell Trabecular meshwork cell Lymphocyte Macrophage Myelinating schwann cell Non-pigmented ciliary epithelial cell Mast cell Ciliary muscle cell Fibroblast Pigmented ciliary epithelial cell Blood vessel endothelial cell Pericyte Endothelial cell B cell Melanocyte

Eye trabecular meshwork Ciliary body

85-year-old stage 16-year-old stage 70-year-old stage 21-year-old stage 32-year-old stage 62-year-old stage 60-year-old stage 64-year-old stage 45-year-old stage 74-year-old stage 65-year-old stage 1-year-old stage 2-year-old stage 75-year-old stage 27-year-old stage 78-year-old stage 56-year-old stage 68-year-old stage 61-year-old stage 34-year-old stage 51-year-old stage 72-year-old stage 73-year-old stage Newborn stage (0-28 days) 69-year-old stage 43-year-old stage 55-year-old stage 11-year-old stage

Normal

Female Male

10x 5' v2 10x 3' v3 10x 3' v2

Cell

Mus musculus

Gabaergic amacrine cell Retinal rod cell Type 6 cone bipolar cell (sensu mus) Astrocyte Retinal cone cell Type 3b cone bipolar cell Type 9 cone bipolar cell (sensu mus) Type 5b cone bipolar cell Type 4 cone bipolar cell (sensu mus) Type 8 cone bipolar cell (sensu mus) Microglial cell Type 2 cone bipolar cell (sensu mus) Type 3a cone bipolar cell Pericyte Endothelial cell Mueller cell Retinal bipolar neuron Type 5 cone bipolar cell (sensu mus) Type 1 cone bipolar cell (sensu mus) Amacrine cell Glycinergic amacrine cell Type 5a cone bipolar cell Retinal pigment epithelial cell Type 7 cone bipolar cell (sensu mus) Retinal ganglion cell Retina horizontal cell Rod bipolar cell

Optic cup Retina

12-week-old stage 4-month-old stage 7-week-old stage 5-week-old stage Postnatal stage 25-week-old stage 2-week-old stage Prime adult stage 9-week-old stage 8-week-old stage 11-week-old stage

Normal

Male

10x 3' v3 Drop-seq 10x 3' v2

Cell

Mus musculus

Gabaergic amacrine cell Retinal rod cell Type 6 cone bipolar cell (sensu mus) Astrocyte Retinal cone cell Type 3b cone bipolar cell Type 9 cone bipolar cell (sensu mus) Type 5b cone bipolar cell Type 4 cone bipolar cell (sensu mus) Type 8 cone bipolar cell (sensu mus) Microglial cell Type 2 cone bipolar cell (sensu mus) Type 3a cone bipolar cell Pericyte Endothelial cell Mueller cell Retinal bipolar neuron Type 5 cone bipolar cell (sensu mus) Type 1 cone bipolar cell (sensu mus) Amacrine cell Glycinergic amacrine cell Type 5a cone bipolar cell Retinal pigment epithelial cell Type 7 cone bipolar cell (sensu mus) Retinal ganglion cell Retina horizontal cell Rod bipolar cell

Normal

10x 3' v3 Drop-seq 10x 3' v2

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron

Middle temporal gyrus

80-year-old stage 85-year-old stage 82-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 60-year-old stage 80 year-old and over stage 89-year-old stage 65-year-old stage 75-year-old stage Adult stage 78-year-old stage 68-year-old stage 72-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 29-year-old stage 87-year-old stage 81-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x 3' v3 10x multiome

Nucleus

Homo sapiens

Pro-b cell Naive b cell Classical monocyte Cd8-positive, alpha-beta memory t cell, cd45ro-positive Non-classical monocyte Lymphocyte Macrophage Naive thymus-derived cd4-positive, alpha-beta t cell Alveolar macrophage Mucosal invariant t cell Effector memory cd4-positive, alpha-beta t cell Mast cell Cd4-positive helper t cell Cd16-negative, cd56-bright natural killer cell, human Erythroid lineage cell Megakaryocyte Progenitor cell Plasmablast Dendritic cell, human Cd8-positive, alpha-beta memory t cell Conventional dendritic cell Naive thymus-derived cd8-positive, alpha-beta t cell Group 3 innate lymphoid cell Alpha-beta t cell Plasma cell Regulatory t cell Memory b cell Plasmacytoid dendritic cell Effector memory cd8-positive, alpha-beta t cell, terminally differentiated Precursor b cell T follicular helper cell Gamma-delta t cell Cd16-positive, cd56-dim natural killer cell, human Germinal center b cell

Blood Ileum Thymus Mesenteric lymph node Liver Spleen Skeletal muscle tissue Caecum Lung Lamina propria Transverse colon Omentum Sigmoid colon Duodenum Bone marrow Jejunal epithelium Thoracic lymph node

Eighth decade stage Seventh decade stage Sixth decade stage

Normal

Female Male

10x 5' v2 10x 5' v1 10x 3' v3

Cell

Homo sapiens

Lymphocyte Stromal cell Mast cell Epithelial cell Hepatocyte Endothelial cell B cell Erythrocyte Plasmacytoid dendritic cell Myeloid cell

Normal Hepatocellular carcinoma Metabolic dysfunction-associated steatohepatitis Alcoholic liver cirrhosis Primary biliary cholangitis

10x 3' v3 10x 3' v2