Homo sapiens

Mature gamma-delta t cell Mucosal invariant t cell Cd8-positive, alpha-beta memory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell Cd8aa(i) thymocyte

Blood

32-year-old stage 57-year-old stage 62-year-old stage 60-year-old stage 64-year-old stage 25-year-old stage 33-year-old stage 65-year-old stage 27-year-old stage 28-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 31-year-old stage 56-year-old stage 58-year-old stage 61-year-old stage 34-year-old stage 35-year-old stage 30-year-old stage 26-year-old stage 12-year-old stage 29-year-old stage 55-year-old stage 11-year-old stage

Normal Cytomegalovirus infection

Female Male

10x 3' v3

Cell

Mus musculus

Glutamatergic neuron Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Gabaergic neuron Macrophage Leptomeningeal cell Microglial cell Pericyte Endothelial cell Smooth muscle cell

Primary motor cortex

Prime adult stage

Normal

Female Male

Smart-seq v4 10x 3' v3 10x 3' v2

Cell Nucleus

Homo sapiens

Endothelial stalk cell Endothelial cell of hepatic sinusoid Macrophage Adipocyte Monocyte Cell of skeletal muscle Blood vessel smooth muscle cell Fibroblast Malignant cell Hepatocyte Blood vessel endothelial cell B cell Hepatic stellate cell Plasma cell Mature nk t cell T cell Neuron Chondrocyte

Skin epidermis Axilla Breast Liver Chest wall Brain Bone spine

50-year-old stage 57-year-old stage 62-year-old stage 46-year-old stage 60-year-old stage 44-year-old stage 40-year-old stage 27-year-old stage 37-year-old stage 28-year-old stage 63-year-old stage 54-year-old stage 31-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 35-year-old stage 39-year-old stage 43-year-old stage 41-year-old stage

Breast cancer

Female

10x 3' v3 10x 3' v2

Nucleus

Homo sapiens

Schwann cell Cardiac muscle cell Lymphocyte Macrophage Mesothelial cell Endocardial cell Adipocyte Cardiac blood vessel endothelial cell Fibroblast Pericyte Smooth muscle cell

Right atrium auricular region

Eighth decade stage Ninth decade stage Fifth decade stage Seventh decade stage Sixth decade stage

Normal Coronary artery disorder Myocardial infarction Heart valve disorder

Female Male

10x 3' v3

Nucleus

Homo sapiens

Mast cell Epithelial cell Neutrophil Megakaryocyte Fibroblast Malignant cell Endothelial cell B cell T cell Plasmacytoid dendritic cell Mononuclear phagocyte

Skin epidermis Breast Colorectum Blood Liver Lung Ovary Uvea

80-year-old stage 85-year-old stage 66-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 86-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 44-year-old stage 64-year-old stage 45-year-old stage 25-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage Eighth decade stage 75-year-old stage 27-year-old stage 37-year-old stage 71-year-old stage Ninth decade stage 78-year-old stage 63-year-old stage 54-year-old stage Fifth decade stage 47-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 51-year-old stage 42-year-old stage 53-year-old stage 72-year-old stage 73-year-old stage Seventh decade stage 69-year-old stage 43-year-old stage 38-year-old stage 41-year-old stage 81-year-old stage 83-year-old stage Sixth decade stage

Large cell carcinoma Ovarian clear cell adenocarcinoma Normal Breast cancer Melanoma Invasive ductal breast carcinoma Uveal melanoma Lung cancer Serous adenocarcinoma Squamous cell carcinoma Pleomorphic carcinoma Lung adenocarcinoma Colorectal adenocarcinoma Colon adenocarcinoma Metaplastic breast carcinoma Rectosigmoid carcinoma Breast apocrine carcinoma Liver cancer Ovarian cancer Cecum adenocarcinoma

Female Male

Smart-seq2 10x 5' v2 10x 3' v3 Indrop 10x 3' v1 10x 3' v2

Cell

Homo sapiens

Cd4-positive, alpha-beta t cell Double-positive, alpha-beta thymocyte Cd8-positive, alpha-beta regulatory t cell Immature alpha-beta t cell Double negative thymocyte Cd8-positive, alpha-beta memory t cell Cd8-positive, alpha-beta t cell Early t lineage precursor Regulatory t cell T cell Natural killer cell Gamma-delta t cell

Thymus

6-month-old stage 3-month-old stage 13th week post-fertilization stage 12th week post-fertilization stage 14-month-old stage 5-month-old stage 2-year-old stage 10-month-old stage 20th week post-fertilization stage 17th week post-fertilization stage 2-month-old stage 15th week post-fertilization stage 14th week post-fertilization stage 24th week post-fertilization stage 18th week post-fertilization stage Newborn stage (0-28 days)

Normal

Female Male

10x 5' v2 10x 5' v1 10x 3' v3 10x 3' v2

Cell

Homo sapiens

Cd1c-positive myeloid dendritic cell Cd141-positive myeloid dendritic cell Axl+ dendritic cell, human Cd14-positive, cd16-negative classical monocyte Plasmacytoid dendritic cell Cd14-low, cd16-positive monocyte

Blood

80-year-old stage 85-year-old stage 82-year-old stage 66-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 80 year-old and over stage 44-year-old stage 48-year-old stage 64-year-old stage 89-year-old stage 45-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage 40-year-old stage 75-year-old stage 71-year-old stage 78-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 47-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 53-year-old stage 72-year-old stage 73-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 87-year-old stage 41-year-old stage 81-year-old stage 55-year-old stage 83-year-old stage

Normal Cytomegalovirus infection

Female Male

10x gene expression flex

Cell

Homo sapiens

Naive b cell Cd4-positive, alpha-beta memory t cell Lymphocyte Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Cd16-negative, cd56-bright natural killer cell, human Plasmablast Naive thymus-derived cd8-positive, alpha-beta t cell Cd8-positive, alpha-beta t cell Plasma cell Regulatory t cell Memory b cell Mature nk t cell, human Natural killer cell Gamma-delta t cell Cd16-positive, cd56-dim natural killer cell, human

Blood

19-year-old stage 18-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 57-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 22-year-old stage 44-year-old stage 48-year-old stage 45-year-old stage 25-year-old stage 33-year-old stage 40-year-old stage 27-year-old stage 23-year-old stage 37-year-old stage 28-year-old stage 36-year-old stage 54-year-old stage 20-year-old stage 47-year-old stage 31-year-old stage 58-year-old stage 34-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 35-year-old stage 53-year-old stage 30-year-old stage 24-year-old stage 26-year-old stage 39-year-old stage 43-year-old stage 29-year-old stage 38-year-old stage 41-year-old stage 55-year-old stage

Normal

Female Male

10x 5' v2

Cell

Mus musculus

Glutamatergic neuron

Orbitofrontal cortex Anterior olfactory nucleus Caudate-putamen Dentate gyrus of hippocampal formation Primary somatosensory cortex Olfactory cortex Lateral septal complex Primary auditory cortex Anterior cerebral artery Pallidum Cerebral cortex Primary motor cortex Hippocampal field Piriform cortex Nucleus accumbens Secondary somatosensory cortex

Prime adult stage

Normal

Male

Scatac-seq

Nucleus

Mus musculus

Hypendymal cell Mural cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Pituitary gland cell Microglial cell Fibroblast Tanycyte Ependymal cell Endothelial cell Erythrocyte Neuron

Hypothalamus

23-day-old stage 4-week-old stage 1-week-old stage 6-week-old stage 26-day-old stage 2-month-old stage Juvenile stage 2-week-old stage 9-week-old stage 8-week-old stage

Normal

Female Male

10x 3' v3 10x 3' v1 Drop-seq 10x 3' v2

Nucleus Cell

Mus musculus

Inhibitory interneuron Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Macrophage Medium spiny neuron Cell Microglial cell Pericyte Endothelial cell T cell Neuron

Brain ventricle Corpus callosum Cortical layer ii/iii Cortical layer vi Pia mater Olfactory region Striatum Cortical layer v

20-month-old stage and over 4-week-old stage 24-week-old stage

Normal

Female

Merfish

Mus musculus

Inhibitory interneuron Vascular leptomeningeal cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Macrophage Medium spiny neuron Cell Microglial cell Pericyte Endothelial cell T cell Neuron

Brain ventricle Corpus callosum Cortical layer ii/iii Cortical layer vi Pia mater Olfactory region Striatum Cortical layer v

20-month-old stage and over 4-week-old stage 24-week-old stage

Normal

Female

Merfish