Homo sapiens

T cell Endothelial cell of venule Natural killer cell Non-myelinating schwann cell Cytotoxic t cell Retinal pigment epithelial cell Mast cell Endothelial cell of arteriole Endothelial cell of artery Schwann cell Melanocyte Classical monocyte Smooth muscle cell Plasmacytoid dendritic cell Macrophage Fenestrated endothelial cell Innate lymphoid cell Helper t cell Plasma cell Vein endothelial cell Megakaryocyte B cell Pericyte Vascular associated smooth muscle cell Fibroblast Myelinating schwann cell Dendritic cell Non-classical monocyte

Chorioretinal region

69-year-old stage 21-year-old stage 34-year-old stage 65-year-old stage 43-year-old stage 71-year-old stage 53-year-old stage 68-year-old stage 30-year-old stage 17-year-old stage 82-year-old stage 50-year-old stage 72-year-old stage 81-year-old stage 16-year-old stage 58-year-old stage 25-year-old stage 90 year-old and over stage 32-year-old stage 77-year-old stage 64-year-old stage 62-year-old stage 86-year-old stage 46-year-old stage 60-year-old stage Newborn stage (0-28 days) 63-year-old stage 47-year-old stage 11-year-old stage 27-year-old stage 10-year-old stage

Normal

Female Male

10x 5' v2 10x 3' v3

Nucleus

Homo sapiens

Neuron

Basal forebrain Brain Caudate-putamen Cerebellar peduncular complex Cerebellum Cerebral subcortex Choroid plexus epithelium Cortex Diencephalon Dorsal plus ventral thalamus Entorhinal cortex Forebrain Frontal cortex Head Hindbrain Hippocampal formation Hypothalamus Lower layers of the cortex Medulla oblongata Metencephalon Midbrain Midbrain tectum Occipital cortex Pons Striatum Telencephalon Upper layers of the cortex

13th week post-fertilization stage 14th week post-fertilization stage 15th week post-fertilization stage 12th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 16 Carnegie stage 15 Carnegie stage 18 11th week post-fertilization stage

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Natural killer cell Cd16-positive, cd56-dim natural killer cell, human Innate lymphoid cell Cd16-negative, cd56-bright natural killer cell, human

Blood

66-year-old stage 69-year-old stage 75-year-old stage 65-year-old stage 42-year-old stage 89-year-old stage 43-year-old stage 87-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 44-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 80-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 80 year-old and over stage 49-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 77-year-old stage 64-year-old stage 45-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage 40-year-old stage

Cytomegalovirus infection Normal

Female Male

10x gene expression flex

Cell

Mus musculus

Cardiac valve cell Spinal cord interneuron Premigratory neural crest cell Paraxial cell Notochordal cell Neuroplacodal cell Neurectodermal cell Hemangioblast Mesodermal cell Gut endothelial cell Primitive red blood cell Splanchnic mesodermal cell Surface ectodermal cell Midbrain dopaminergic neuron Endodermal cell Anterior visceral endoderm cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Bladder urothelial cell Podocyte Conjunctiva goblet cell Epithelial cell of parathyroid gland Gingival epithelial cell Olfactory epithelial cell Endo-epithelial cell Intermediate mesodermal cell Corneal epithelial cell Pancreatic acinar cell Ectodermal cell Auditory epithelial cell Kidney collecting duct intercalated cell Metanephric mesenchyme stem cell Epithelial cell of amnion Ureteric bud cell Notochordal cell Epithelial cell of proximal tubule Epithelial cell Epithelial cell of thyroid gland Kidney distal convoluted tubule epithelial cell Kidney collecting duct principal cell Kidney connecting tubule epithelial cell Keratinocyte Pituitary gland cell Kidney epithelial cell Lens epithelial cell Kidney loop of henle ascending limb epithelial cell Epithelial cell of thymus Primordial germ cell Intrahepatic cholangiocyte

Embryo

Theiler stage 22 Theiler stage 27 Theiler stage 24 Theiler stage 18 Theiler stage 25 Theiler stage 14 Theiler stage 16 Theiler stage 17 Theiler stage 15 Theiler stage 19 Theiler stage 26 Theiler stage 13 Theiler stage 20 Theiler stage 12 Theiler stage 21 Theiler stage 23

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Radial glial cell

Basal forebrain Brain Caudate-putamen Cerebellar peduncular complex Cerebellum Cerebral subcortex Choroid plexus epithelium Cortex Diencephalon Dorsal plus ventral thalamus Entorhinal cortex Forebrain Frontal cortex Head Hindbrain Hippocampal formation Hypothalamus Lower layers of the cortex Medulla oblongata Metencephalon Midbrain Midbrain tectum Occipital cortex Pons Striatum Telencephalon Upper layers of the cortex

13th week post-fertilization stage 14th week post-fertilization stage 15th week post-fertilization stage 12th week post-fertilization stage Carnegie stage 20 9th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 Carnegie stage 16 Carnegie stage 15 Carnegie stage 18 11th week post-fertilization stage

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Endothelial cell Hematopoietic precursor cell Lymphocyte Hematopoietic multipotent progenitor cell B cell Monocyte Antibody secreting cell Dendritic cell

Blood

66-year-old stage 69-year-old stage 37-year-old stage 75-year-old stage 34-year-old stage 65-year-old stage 26-year-old stage 89-year-old stage 43-year-old stage 93-year-old stage 71-year-old stage 85-year-old stage 61-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 30-year-old stage 39-year-old stage 44-year-old stage 38-year-old stage 55-year-old stage 84-year-old stage 92-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 80-year-old stage 28-year-old stage 51-year-old stage 57-year-old stage 72-year-old stage 81-year-old stage 49-year-old stage 23-year-old stage 58-year-old stage 59-year-old stage 25-year-old stage 67-year-old stage 32-year-old stage 77-year-old stage 33-year-old stage 62-year-old stage 86-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 31-year-old stage 36-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 73-year-old stage 83-year-old stage 40-year-old stage

Hematologic disorder Myelodysplastic/myeloproliferative disease Normal Myelodysplastic syndrome

Female Male

10x 3' v3

Cell

Drosophila melanogaster

Adult somatic muscle cell of head Indirect flight muscle cell Subperineurial glial cell Visceral muscle cell Eo sensory organ accessory cell Adult renal stem cell Hemocyte Enteroendocrine cell Follicle cell Epithelial cell Germline cell Adult alary muscle cell Muscle cell Escort cell Ovarian sheath muscle cell Embryonic/larval crystal cell Mechanosensory neuron Sensory neuron Adult brain perineurial glial cell Cell body glial cell Adult heart ventral longitudinal muscle cell

Heart Trachea Body wall Fat body Gut Adult oenocyte Antenna Haltere Head Leg Ovary Testis Wing Malpighian tubule Body proper Male reproductive gland Insect proboscis

Day 3 of adulthood Day 5 of adulthood Day 2 of adulthood Day 1 of adulthood

Normal

Mixed sex Female Male

10x 3' v3

Rattus norvegicus

Cardiac pacemaker cell of sinoatrial node Myocyte of atrioventricular node T cell Brown adipocyte Club cell Cycling t cell Cardiac atrium fibroblast Cardiac endothelial cell Natural killer cell Endothelial cell of lymphatic vessel Non-myelinating schwann cell Eukaryotic cell Endothelial cell Mast cell Cd8-positive, alpha-beta t cell Peripheral nervous system neuron Schwann cell Cardiac neuron Macrophage Myocyte of sinoatrial node Cardiac ventricle fibroblast Mesothelial cell of epicardium Cd14-low, cd16-positive monocyte B cell Pericyte Regular atrial cardiac myocyte Monocyte Vascular associated smooth muscle cell Regular ventricular cardiac myocyte Fibroblast Dendritic cell Fibroblast of cardiac tissue White adipocyte

Normal

10x 3' v2

Homo sapiens

Endothelial cell Hematopoietic precursor cell Lymphocyte Hematopoietic multipotent progenitor cell B cell Monocyte Antibody secreting cell Dendritic cell

Blood

66-year-old stage 69-year-old stage 37-year-old stage 75-year-old stage 91-year-old stage 34-year-old stage 65-year-old stage 26-year-old stage 89-year-old stage 43-year-old stage 71-year-old stage 85-year-old stage 53-year-old stage 61-year-old stage 88-year-old stage 52-year-old stage 54-year-old stage 68-year-old stage 48-year-old stage 39-year-old stage 56-year-old stage 55-year-old stage 84-year-old stage 82-year-old stage 50-year-old stage 90-year-old stage 80-year-old stage 51-year-old stage 72-year-old stage 81-year-old stage 74-year-old stage 58-year-old stage 59-year-old stage 67-year-old stage 32-year-old stage 77-year-old stage 64-year-old stage 94-year-old stage 45-year-old stage 62-year-old stage 41-year-old stage 70-year-old stage 46-year-old stage 60-year-old stage 78-year-old stage 31-year-old stage 63-year-old stage 47-year-old stage 76-year-old stage 79-year-old stage 73-year-old stage 83-year-old stage 27-year-old stage 40-year-old stage

Hematologic disorder Myelodysplastic/myeloproliferative disease Normal Myelodysplastic syndrome

Female Male

10x 3' v3

Cell

Homo sapiens

Oligodendrocyte

Brodmann (1909) area 13 Brodmann (1909) area 14 Brodmann (1909) area 19 Brodmann (1909) area 23 Brodmann (1909) area 24 Brodmann (1909) area 25 Brodmann (1909) area 32 Brodmann (1909) area 35 Brodmann (1909) area 38 Brodmann (1909) area 40 Brodmann (1909) area 43 Brodmann (1909) area 46 Accessory basal amygdaloid nucleus Agranular insular cortex Anterior cortical amygdaloid nucleus Anterior nuclear group Anterior olfactory nucleus Basolateral amygdaloid nuclear complex Bed nucleus of stria terminalis Body of caudate nucleus Central amygdaloid nucleus Centromedian nucleus of thalamus Cerebellar nuclear complex Cerebellar vermis Cerebrocerebellum Claustrum of brain Corticomedial nuclear complex Cuneus cortex Dentate gyrus of hippocampal formation Dorsal tegmental nucleus Epithalamus Extrastriate cortex Globus pallidus Granular insular cortex Gray matter of midbrain Hippocampal field Hypothalamus Inferior frontal gyrus Inferior olivary complex Inferior temporal gyrus Intralaminar nuclear group Lateral amygdaloid nucleus Lateral entorhinal cortex Lateral geniculate body Lateral nuclear group of thalamus Lateral posterior nucleus of thalamus Mammillary body Medial dorsal nucleus of thalamus Medial entorhinal cortex Medial geniculate body Medial zone of hypothalamus Median tuberal portion Midbrain nucleus Midbrain tectum Middle temporal gyrus Nucleus accumbens Nucleus of dorsal thalamus Nucleus of medulla oblongata Parabrachial nucleus Perirhinal cortex Piriform cortex Pontine nuclear group Pontine reticular formation Pontine tegmentum Posterior parahippocampal gyrus Preoptic area Pretectal region Primary auditory cortex Primary motor cortex Primary somatosensory cortex Primary visual cortex Pulvinar nucleus Putamen Red nucleus Retrosplenial region Rostral ca3 Septal nuclear complex Spinal cord Subicular complex Substantia innominata Substantia nigra Subthalamic nucleus Superior parietal cortex Superior temporal gyrus Supraoptic nucleus Temporal fusiform gyrus Uncal ca1 Ventral anterior nucleus of thalamus

42-year-old stage 29-year-old stage 50-year-old stage 60-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Cd14-positive, cd16-positive monocyte Natural killer cell Endothelial cell of lymphatic vessel Activated cd4-positive, alpha-beta t cell Endothelial cell Mature nk t cell Mast cell Endothelial cell of artery Capillary endothelial cell Smooth muscle cell Neural cell Cd4-positive, alpha-beta cytotoxic t cell Cd8-positive, alpha-beta cytotoxic t cell Macrophage Activated cd8-positive, alpha-beta t cell Vein endothelial cell Cd14-positive monocyte Mesothelial cell B cell Pericyte Epicardial adipocyte Regular atrial cardiac myocyte Monocyte Regular ventricular cardiac myocyte Fibroblast Dendritic cell

Apex of heart Heart left ventricle Heart right ventricle Interventricular septum Left cardiac atrium Right cardiac atrium

Sixth decade stage Fifth decade stage Seventh decade stage Eighth decade stage

Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus Cell