Homo sapiens

L6b glutamatergic cortical neuron

Dorsolateral prefrontal cortex

42-year-old stage 68-year-old stage 88-year-old stage 86-year-old stage 82-year-old stage 83-year-old stage 75-year-old stage 80-year-old stage 77-year-old stage 89-year-old stage 78-year-old stage 72-year-old stage 80 year-old and over stage 81-year-old stage 87-year-old stage 84-year-old stage 85-year-old stage 50-year-old stage 29-year-old stage 69-year-old stage 70-year-old stage

Dementia Normal

Female Male

10x multiome 10x 3' v3

Nucleus

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Macula densa epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

6-month-old stage

Autosomal dominant polycystic kidney disease

Male

Slide-seqv2

Homo sapiens

Astrocyte

White matter of parietal lobe Prefrontal cortex White matter of frontal lobe White matter of occipital lobe White matter of temporal lobe

68-year-old stage 82-year-old stage 83-year-old stage 76-year-old stage 72-year-old stage 81-year-old stage 53-year-old stage 70-year-old stage

Alzheimer disease Leukoencephalopathy, diffuse hereditary, with spheroids 1 Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Near-projecting glutamatergic cortical neuron

Anterior cortical amygdaloid nucleus Temporal fusiform gyrus Posterior parahippocampal gyrus Cuneus cortex Brodmann (1909) area 23 Subicular complex Brodmann (1909) area 32 Superior temporal gyrus Brodmann (1909) area 13 Middle temporal gyrus Brodmann (1909) area 19 Granular insular cortex Hippocampal field Brodmann (1909) area 43 Supraoptic nucleus Agranular insular cortex Lateral posterior nucleus of thalamus Primary visual cortex Superior parietal cortex Bed nucleus of stria terminalis Primary auditory cortex Primary motor cortex Medial zone of hypothalamus Brodmann (1909) area 14 Nucleus accumbens Hypothalamus Primary somatosensory cortex Extrastriate cortex Accessory basal amygdaloid nucleus Brodmann (1909) area 25 Retrosplenial region Brodmann (1909) area 40 Central amygdaloid nucleus Preoptic area Claustrum of brain Lateral amygdaloid nucleus Midbrain tectum Piriform cortex Anterior olfactory nucleus Brodmann (1909) area 46 Brodmann (1909) area 38 Substantia innominata Brodmann (1909) area 24 Inferior frontal gyrus Perirhinal cortex Septal nuclear complex Inferior temporal gyrus Lateral entorhinal cortex

42-year-old stage 60-year-old stage 50-year-old stage 29-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Regulatory t cell B cell Malignant cell Cd8-positive, alpha-beta t cell Epithelial cell Fibroblast Plasma cell Myeloid cell Cd4-positive helper t cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Natural killer cell Myoepithelial cell Dendritic cell B cell Neutrophil Salivary gland glandular cell Macrophage Mural cell Cd8-positive, alpha-beta t cell Keratinocyte Blood vessel endothelial cell Ionocyte Fibroblast Plasma cell Cd4-positive helper t cell Acinar cell of salivary gland

Parotid gland Submandibular gland

62-year-old stage Fourth decade stage 59-year-old stage 48-year-old stage Sixth decade stage 69-year-old stage

Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Renal medulla

65-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Oligodendrocyte Natural killer cell Astrocyte T cell Medial ganglionic eminence derived interneuron Pvalb chandelier gabaergic interneuron Microglial cell Oligodendrocyte precursor cell Neuron Ependymal cell Pericyte Committed oligodendrocyte precursor Monocyte Intratelencephalic-projecting glutamatergic cortical neuron Sst chodl gabaergic interneuron Hippocampal ca1-3 neuron Endothelial cell Amygdala excitatory neuron Caudal ganglionic eminence derived interneuron Thalamic excitatory neuron Choroid plexus epithelial cell Central nervous system macrophage Midbrain-derived inhibitory neuron Fibroblast Lamp5 lhx6 neuron Mammillary body neuron Near-projecting glutamatergic cortical neuron

Hypothalamus

42-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Spinal cord interneuron Paraxial cell Gut endothelial cell Hemangioblast Cardiac valve cell Endodermal cell Primitive red blood cell Midbrain dopaminergic neuron Anterior visceral endoderm cell Premigratory neural crest cell Mesodermal cell Neuroplacodal cell Surface ectodermal cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

13-week-old stage

Normal

Male

Slide-seqv2

Mus musculus

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney proximal convoluted tubule epithelial cell Podocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Mesangial cell Kidney granular cell Kidney distal convoluted tubule epithelial cell

Kidney

13-week-old stage

Diabetic kidney disease

Male

Slide-seqv2

Homo sapiens

Cortical thymic epithelial cell Medullary thymic epithelial cell Epithelial cell of thymus

Thymus

10th week post-fertilization stage Organogenesis stage Fourth decade stage 9th week post-fertilization stage 16th week post-fertilization stage 11th week post-fertilization stage 15th week post-fertilization stage 15-19 year-old 13th week post-fertilization stage 12th week post-fertilization stage 17th week post-fertilization stage 3-month-old stage

Normal

Female Male

10x 3' v2 10x 5' v1 10x 3' v3

Cell