Drosophila melanogaster

Male germline cell Adult neuron Neuron Tracheolar cell Neuron Muscle cell Fat cell Adult enterocyte Epithelial cell Female germline cell Hemocyte Sensory neuron Adult oenocyte Oenocyte Somatic precursor cell Motor neuron Cardial cell Glial cell Adult tracheocyte Adult alary muscle cell

Oenocyte

Day 5 of adulthood

Normal

Female Male

10x 3' v3

Homo sapiens

Oligodendrocyte Astrocyte Leukocyte Microglial cell Oligodendrocyte precursor cell Progenitor cell Neuron Pericyte Macrophage Vascular associated smooth muscle cell Endothelial cell

Brain Brain white matter

49-year-old stage 64-year-old stage 82-year-old stage 57-year-old stage 60-year-old stage 35-year-old stage 44-year-old stage 37-year-old stage

Multiple sclerosis Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Natural killer cell Melanocyte T cell Sebocyte Regulatory t cell B cell Neutrophil Keratocyte Muscle cell Mature nk t cell Smooth muscle cell Cd4-positive, alpha-beta t cell Macrophage Myeloid dendritic cell Cd8-positive, alpha-beta t cell Erythrocyte Stromal cell Endothelial cell Activated cd4-positive, alpha-beta t cell Basophil Epithelial cell Fibroblast Plasma cell Langerhans cell Mast cell

Skin of abdomen Skin of chest Skin of body Buccal mucosa

59-year-old stage 33-year-old stage 61-year-old stage 45-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Kidney distal convoluted tubule epithelial cell

Renal medulla

53-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney interstitial fibroblast Leukocyte Kidney collecting duct principal cell Macrophage Blood vessel smooth muscle cell Endothelial cell Kidney collecting duct intercalated cell Kidney distal convoluted tubule epithelial cell

Renal medulla

53-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Oligodendrocyte Natural killer cell Astrocyte T cell Pvalb chandelier gabaergic interneuron Microglial cell Oligodendrocyte precursor cell Neuron Pericyte Committed oligodendrocyte precursor Monocyte Vascular associated smooth muscle cell Endothelial cell Caudal ganglionic eminence derived interneuron Thalamic excitatory neuron Central nervous system macrophage Midbrain-derived inhibitory neuron Fibroblast Lamp5 lhx6 neuron

Nucleus of dorsal thalamus

50-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Notochordal cell Spinal cord interneuron Paraxial cell Gut endothelial cell Splanchnic mesodermal cell Hemangioblast Cardiac valve cell Endodermal cell Primitive red blood cell Midbrain dopaminergic neuron Anterior visceral endoderm cell Premigratory neural crest cell Mesodermal cell Neuroplacodal cell Surface ectodermal cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Homo sapiens

Intestine goblet cell Transit amplifying cell of small intestine Intestinal crypt stem cell of large intestine Progenitor cell Enterocyte Secretory cell Intestinal crypt stem cell of small intestine Enteroendocrine cell Transit amplifying cell of colon Gut absorptive cell

Hindgut Colon Ileum

10th week post-fertilization stage 14th week post-fertilization stage 16th week post-fertilization stage 11th week post-fertilization stage 21st week post-fertilization stage Carnegie stage 22 20th week post-fertilization stage 23rd week post-fertilization stage 19th week post-fertilization stage 13th week post-fertilization stage 18th week post-fertilization stage 17th week post-fertilization stage

Normal

Female Male

10x 3' v3

Cell

Mus musculus

Notochordal cell Spinal cord interneuron Paraxial cell Gut endothelial cell Splanchnic mesodermal cell Hemangioblast Cardiac valve cell Endodermal cell Primitive red blood cell Midbrain dopaminergic neuron Anterior visceral endoderm cell Premigratory neural crest cell Mesodermal cell Neuroplacodal cell Surface ectodermal cell

Embryo

Theiler stage 14

Normal

Slide-seqv2

Homo sapiens

Malignant cell Epithelial cell Fibroblast Myeloid cell

Kidney

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Danio rerio

Splanchnic mesodermal cell Stromal cell Mesodermal cell Mesothelial cell

Heart primordium Pronephric mesoderm Proepicardium Anterior lateral plate mesoderm Lateral plate mesoderm

Segmentation:14-19 somites Hatching:pec-fin Larval:protruding-mouth Pharyngula:prim-5 Pharyngula:high-pec Hatching:long-pec Segmentation:20-25 somites Segmentation:26+ somites Pharyngula:prim-15 Pharyngula:prim-25

Normal

Sci-rna-seq3

Nucleus

Mus musculus

Notochordal cell Spinal cord interneuron Paraxial cell Gut endothelial cell Splanchnic mesodermal cell Hemangioblast Cardiac valve cell Endodermal cell Primitive red blood cell Midbrain dopaminergic neuron Premigratory neural crest cell Mesodermal cell Neuroplacodal cell Surface ectodermal cell

Embryo

Theiler stage 14

Normal

Slide-seqv2