Homo sapiens

Trophoblast giant cell Extravillous trophoblast Syncytiotrophoblast cell Placental villous trophoblast

Decidua basalis

9th week post-fertilization stage

Normal

Female

10x 3' v3 10x multiome

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Brodmann (1909) area 38

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Cd4-positive helper t cell Malignant cell B cell Cd8-positive, alpha-beta t cell Plasma cell Regulatory t cell Exhausted t cell Myeloid cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Cd4-positive helper t cell Malignant cell B cell Cd8-positive, alpha-beta t cell Plasma cell Regulatory t cell Exhausted t cell Myeloid cell

Lung

61-year-old stage

Renal cell carcinoma

Male

Slide-seqv2

Homo sapiens

Lung resident memory cd4-positive, alpha-beta t cell Innate lymphoid cell Lung resident memory cd8-positive, alpha-beta t cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd8-positive, alpha-beta t cell Effector cd4-positive, alpha-beta t cell Regulatory t cell Natural killer cell Gamma-delta t cell

Lung Bronchus Upper lobe of left lung Trachea Lower lobe of left lung

66-year-old stage 64-year-old stage 75-year-old stage 59-year-old stage 58-year-old stage 35-year-old stage Seventh decade stage 29-year-old stage Sixth decade stage

Normal

Female Male

10x 3' v3 10x 5' transcription profiling 10x 3' v2

Cell Nucleus

Homo sapiens

Transit amplifying cell Corneal epithelial cell Leukocyte Basal cell Fibroblast Corneal endothelial cell

Cornea

66-year-old stage 33-year-old stage 65-year-old stage 47-year-old stage 30-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Mesothelial cell Malignant cell

Pleural effusion

Adult stage

Triple-negative breast carcinoma Luminal b breast carcinoma

10x 3' v3 10x 3' v2

Cell

Danio rerio

Eye photoreceptor cell Retinal progenitor cell Retinal bipolar neuron Amacrine cell Retinal pigment epithelial cell Retinal ganglion cell Purkinje cell Retina horizontal cell

Ganglionic layer of retina Inner nuclear layer of retina Pigmented layer of retina Cerebellum Retina Outer nuclear layer of retina

Pharyngula:high-pec Segmentation:26+ somites Pharyngula:prim-5 Hatching:pec-fin Hatching:long-pec Segmentation:20-25 somites Segmentation:14-19 somites Larval:protruding-mouth Pharyngula:prim-15 Pharyngula:prim-25

Normal

Sci-rna-seq3

Nucleus

Callithrix jacchus

Endothelial cell

Putamen Ventral thalamus Posterior hypothalamic region Secondary visual cortex Hippocampal formation Lateral geniculate body Primary somatosensory cortex Lateral nuclear group of thalamus Brodmann (1909) area 23 Temporal cortex Central amygdaloid nucleus Dorsal thalamus Medial amygdaloid nucleus Primary auditory cortex Dorsolateral prefrontal cortex Thalamic complex Temporal lobe Brodmann (1909) area 25 Parietal cortex Primary motor cortex Amygdala Primary visual cortex Lateral amygdaloid nucleus Anterior hypothalamic region Ventral lateral nucleus of thalamus Caudate nucleus Medial dorsal nucleus of thalamus Nucleus accumbens Basal forebrain Brainstem Prefrontal cortex

Prime adult stage Late adult stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Cd4-positive helper t cell Malignant cell B cell Cd8-positive, alpha-beta t cell Plasma cell Regulatory t cell T follicular helper cell Exhausted t cell Myeloid cell

Lymph node

Adult stage

Metastatic melanoma

Slide-seqv2

Drosophila melanogaster

Hemocyte

Antenna

Day 5 of adulthood

Normal

Female Male Mixed sex

10x 3' v3

Mus musculus

Cardiac muscle cell Splanchnic mesodermal cell

Embryo

Theiler stage 23 Theiler stage 24 Theiler stage 21 Theiler stage 13 Theiler stage 25 Theiler stage 17 Theiler stage 16 Theiler stage 26 Theiler stage 12 Theiler stage 18 Theiler stage 20 Theiler stage 19 Theiler stage 27 Theiler stage 14 Theiler stage 15 Theiler stage 22

Normal

Female Male

Sci-rna-seq3

Nucleus