Macaca mulatta

Basket cell

Cerebellar vermis Entorhinal cortex Brodmann (1909) area 19 Inferior temporal gyrus Hippocampal formation Cerebrocerebellum Lateral geniculate body Primary somatosensory cortex Perirhinal cortex Corpus callosum Anterior cingulate cortex Primary auditory cortex Dorsolateral prefrontal cortex Pons Primary motor cortex Superior temporal sulcus Midbrain Amygdala Primary visual cortex Superior parietal cortex Medulla oblongata Inferior parietal cortex Ventral lateral nucleus of thalamus Medial dorsal nucleus of thalamus Nucleus accumbens Head of caudate nucleus Prefrontal cortex

Prime adult stage Juvenile stage

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Lamp5 gabaergic interneuron

Middle temporal gyrus

80-year-old stage 85-year-old stage 82-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 60-year-old stage 80 year-old and over stage 89-year-old stage 65-year-old stage 75-year-old stage Adult stage 78-year-old stage 68-year-old stage 72-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 29-year-old stage 87-year-old stage 81-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x 3' v3 10x multiome

Nucleus

Mus musculus

Urethra urothelial cell Luminal cell of prostate epithelium Basal cell of prostate epithelium Seminal vesicle glandular cell

Urethra Prostate gland

2-month-old stage

Normal

Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Schwann cell Transit amplifying cell Smooth muscle cell of sphincter of pupil Corneal epithelial cell Endothelial cell of lymphatic vessel Leukocyte Lymphocyte Macrophage Non-pigmented ciliary epithelial cell Mast cell Basal cell Ciliary muscle cell Pigmented epithelial cell Fibroblast Pigmented ciliary epithelial cell Blood vessel endothelial cell Pericyte Endothelial cell Corneal endothelial cell Conjunctival epithelial cell Melanocyte Goblet cell

Eye trabecular meshwork Ciliary body Cornea Corneo-scleral junction Iris

66-year-old stage 33-year-old stage 65-year-old stage Adult stage 78-year-old stage 47-year-old stage 30-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Fibroblast Ependymal cell Pericyte Endothelial cell Neuron Central nervous system macrophage

Brodmann (1909) area 43

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus

Olfactory receptor cell Efferent neuron

Embryo

Theiler stage 23 Theiler stage 24 Theiler stage 21 Theiler stage 25 Theiler stage 17 Theiler stage 16 Theiler stage 26 Theiler stage 18 Theiler stage 20 Theiler stage 19 Theiler stage 27 Theiler stage 14 Theiler stage 15 Theiler stage 22

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Capillary endothelial cell Cd8-positive, alpha-beta thymocyte Innate lymphoid cell Endothelial cell of lymphatic vessel Cd4-positive, alpha-beta t cell Macrophage Naive thymus-derived cd4-positive, alpha-beta t cell Mesothelial cell Medullary thymic epithelial cell Neuro-medullary thymic epithelial cell Mast cell Cd4-positive, alpha-beta thymocyte Thymic fibroblast type 2 Neutrophil Monocyte Myo-medullary thymic epithelial cell Vein endothelial cell Vascular associated smooth muscle cell Fibroblast Naive thymus-derived cd8-positive, alpha-beta t cell Hematopoietic precursor cell Endothelial cell B cell Thymocyte Cd8-positive, alpha-beta t cell Plasma cell Erythrocyte Thymic fibroblast type 1 T follicular helper cell Smooth muscle cell Natural killer cell Endothelial cell of artery Fast muscle cell

Thymus

60-year-old stage 45-year-old stage 59-year-old stage 56-year-old stage 61-year-old stage

Normal

Female Male

Smart-seq2 10x 5' v2 10x 3' v3

Cell

Danio rerio

Muscle precursor cell Muscle cell Mesodermal cell Adaxial cell Tendon cell Myoblast Endothelial cell Mesenchymal cell

Hypaxial musculature Presomitic mesoderm Pharyngeal arch Multicellular anatomical structure Somite Myotome Craniocervical muscle

Pharyngula:high-pec Segmentation:26+ somites Pharyngula:prim-5 Hatching:pec-fin Hatching:long-pec Segmentation:20-25 somites Segmentation:14-19 somites Larval:protruding-mouth Pharyngula:prim-15 Pharyngula:prim-25

Normal

Sci-rna-seq3

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Ependymal cell Pericyte Endothelial cell Neuron Central nervous system macrophage

Central amygdaloid nucleus

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Capillary endothelial cell Classical monocyte Endothelial cell of lymphatic vessel Cd4-positive, alpha-beta t cell Non-classical monocyte Macrophage Mast cell Neutrophil Monocyte Myeloid dendritic cell Vein endothelial cell Fibroblast Pericyte Endothelial cell Intermediate monocyte B cell Cd8-positive, alpha-beta t cell Plasma cell Erythrocyte Mature nk t cell T cell Smooth muscle cell Basophil Natural killer cell Endothelial cell of artery

Coronary artery Vasculature Inferior vena cava Abdominal aorta Aorta Left coronary artery Thoracic aorta

60-year-old stage 45-year-old stage 59-year-old stage 56-year-old stage 61-year-old stage

Normal

Female Male

Smart-seq2 10x 3' v3

Cell

Homo sapiens

Endothelial cell of lymphatic vessel Leukocyte Stromal cell Epithelial cell Blood vessel endothelial cell Theca cell Granulosa cell Smooth muscle cell

Ovary

52-year-old stage 27-year-old stage 23-year-old stage 28-year-old stage 54-year-old stage 51-year-old stage 49-year-old stage 29-year-old stage

Normal

Female

10x 3' v3

Nucleus

Homo sapiens

Glutamatergic neuron Mature astrocyte Oligodendrocyte precursor cell Oligodendrocyte Gabaergic neuron Mature microglial cell Endothelial cell

Entorhinal cortex

82-year-old stage 77-year-old stage 50-year-old stage 60-year-old stage 80 year-old and over stage 71-year-old stage 72-year-old stage 87-year-old stage

Normal Alzheimer disease

Male

10x 3' v2

Nucleus