Homo sapiens

Activated cd8-positive, alpha-beta t cell Macrophage Cd14-positive, cd16-positive monocyte Mast cell Monocyte Cd8-positive, alpha-beta cytotoxic t cell B cell Cd4-positive, alpha-beta cytotoxic t cell Activated cd4-positive, alpha-beta t cell Dendritic cell Mature nk t cell Natural killer cell

Interventricular septum Apex of heart Heart left ventricle Left cardiac atrium Right cardiac atrium Heart right ventricle

Eighth decade stage Fifth decade stage Seventh decade stage Sixth decade stage

Normal

Female Male

10x 3' v3 10x 3' v2

Nucleus Cell

Homo sapiens

Kupffer cell Non-classical monocyte Macrophage Monocyte Conventional dendritic cell Myeloid leukocyte Plasmacytoid dendritic cell

Liver

66-year-old stage 77-year-old stage 46-year-old stage 75-year-old stage Adult stage 28-year-old stage 49-year-old stage 53-year-old stage 72-year-old stage 73-year-old stage 55-year-old stage

Normal

Female Male

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Bergmann glial cell Vascular associated smooth muscle cell Fibroblast Ependymal cell Pericyte Endothelial cell Neuron Central nervous system macrophage Choroid plexus epithelial cell

Septal nuclear complex

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Ciliated epithelial cell Endothelial cell of lymphatic vessel Macrophage Stromal cell Mast cell Pericyte Endothelial cell B cell Secretory cell Smooth muscle cell Natural killer cell

Fimbria of fallopian tube Isthmus of fallopian tube Ampulla of fallopian tube

62-year-old stage 65-year-old stage 61-year-old stage 55-year-old stage

Normal

Female

10x scatac-seq

Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage

Posterior parahippocampal gyrus

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Mus musculus Tupaia belangeri

Neuron

Normal

10x 3' v3

Homo sapiens

Alveolar macrophage Metallothionein-positive alveolar macrophage Lung interstitial macrophage Monocyte Cd14-positive monocyte Conventional dendritic cell Lung macrophage Dendritic cell Plasmacytoid dendritic cell

Lung Bronchus Upper lobe of left lung Trachea Lower lobe of left lung

66-year-old stage 64-year-old stage 75-year-old stage 59-year-old stage 58-year-old stage 35-year-old stage Seventh decade stage 29-year-old stage Sixth decade stage

Normal

Female Male

10x 3' v3 10x 5' transcription profiling 10x 3' v2

Cell Nucleus

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Leukocyte Vascular associated smooth muscle cell Fibroblast Pericyte Endothelial cell Neuron Central nervous system macrophage Choroid plexus epithelial cell

Superior temporal gyrus

50-year-old stage 42-year-old stage 29-year-old stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Kidney connecting tubule epithelial cell Leukocyte Kidney distal convoluted tubule epithelial cell Kidney interstitial fibroblast Kidney collecting duct principal cell Kidney loop of henle thin descending limb epithelial cell Blood vessel smooth muscle cell Kidney collecting duct intercalated cell Podocyte Pericyte Endothelial cell Kidney loop of henle epithelial cell Mesangial cell Kidney proximal convoluted tubule epithelial cell

Cortex of kidney

62-year-old stage

Normal

Male

Slide-seqv2

Homo sapiens

Common myeloid progenitor Promonocyte Granulocyte monocyte progenitor cell Promyelocyte Megakaryocyte-erythroid progenitor cell Fraction a pre-pro b cell Megakaryocyte Double negative thymocyte Early lymphoid progenitor Hematopoietic stem cell Hematopoietic multipotent progenitor cell

Thymus Mesenteric lymph node Liver Spleen Kidney Bone marrow Gut wall Yolk sac Skin of body

9th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 11th week post-fertilization stage 17th week post-fertilization stage 15th week post-fertilization stage Carnegie stage 19 Carnegie stage 13 14th week post-fertilization stage 16th week post-fertilization stage Carnegie stage 23 Carnegie stage 20

Normal

Female Male

10x 5' v1 10x 3' v2

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Myofibroblast cell Capillary endothelial cell Classical monocyte Kidney connecting tubule epithelial cell Cd4-positive, alpha-beta t cell Non-classical monocyte Kidney interstitial fibroblast Mast cell Neutrophil Vasa recta ascending limb cell Renal beta-intercalated cell Renal principal cell Kidney resident macrophage Progenitor cell Epithelial cell of proximal tubule Renal intercalated cell Podocyte Urothelial cell B cell Cd8-positive, alpha-beta t cell Kidney epithelial cell Dendritic cell Mature nk t cell Vasa recta descending limb cell Plasmacytoid dendritic cell Renal alpha-intercalated cell Glomerular endothelial cell Natural killer cell

Kidney blood vessel Renal medulla Cortex of kidney Renal pelvis Kidney

70-year-old stage 19-month-old stage 44-year-old stage 64-year-old stage 67-year-old stage 2-year-old stage 63-year-old stage 49-year-old stage 53-year-old stage 72-year-old stage 12-year-old stage 4-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Kidney loop of henle thick ascending limb epithelial cell Myofibroblast cell Kidney connecting tubule epithelial cell Cd4-positive, alpha-beta t cell Lymphocyte Mast cell Neutrophil Vasa recta ascending limb cell Renal beta-intercalated cell Renal principal cell Progenitor cell Epithelial cell of proximal tubule Fibroblast Renal intercalated cell Urothelial cell Epithelial cell of glomerular capsule B cell Cd8-positive, alpha-beta t cell Kidney epithelial cell Peritubular capillary endothelial cell Vasa recta descending limb cell Plasmacytoid dendritic cell Renal alpha-intercalated cell Glomerular endothelial cell Natural killer cell Mononuclear phagocyte

Kidney blood vessel Renal medulla Cortex of kidney Renal pelvis Kidney

70-year-old stage 19-month-old stage 44-year-old stage 64-year-old stage 67-year-old stage 2-year-old stage 63-year-old stage 49-year-old stage 53-year-old stage 72-year-old stage 12-year-old stage 4-year-old stage

Normal

Female Male

10x 3' v2

Cell