Homo sapiens

Classical monocyte Cd4-positive, alpha-beta t cell Non-classical monocyte Lymphocyte Progenitor cell Plasmablast Conventional dendritic cell B cell Cd8-positive, alpha-beta t cell Plasmacytoid dendritic cell Natural killer cell

Blood

66-year-old stage 70-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 57-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 22-year-old stage 44-year-old stage 48-year-old stage 64-year-old stage 45-year-old stage 25-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 40-year-old stage 75-year-old stage 27-year-old stage 23-year-old stage 37-year-old stage 71-year-old stage 28-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 20-year-old stage 47-year-old stage 31-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 34-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 35-year-old stage 53-year-old stage 73-year-old stage 30-year-old stage 24-year-old stage 26-year-old stage 39-year-old stage 69-year-old stage 43-year-old stage 29-year-old stage 38-year-old stage 41-year-old stage 55-year-old stage 83-year-old stage

Normal Systemic lupus erythematosus

Female Male

10x 3' v2

Cell

Homo sapiens

Peripheral blood mononuclear cell Naive b cell Innate lymphoid cell Cd4-positive, alpha-beta t cell Platelet Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd4-positive, alpha-beta t cell Transitional stage b cell Cd16-negative, cd56-bright natural killer cell, human Cd14-positive monocyte Double negative thymocyte Plasmablast Conventional dendritic cell Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Hematopoietic precursor cell Central memory cd8-positive, alpha-beta t cell Cd8-positive, alpha-beta t cell Cd4-positive, alpha-beta cytotoxic t cell Dendritic cell Erythrocyte Regulatory t cell Memory b cell Plasmacytoid dendritic cell Cd14-low, cd16-positive monocyte Natural killer cell Gamma-delta t cell

Blood

80-year-old stage 85-year-old stage 91-year-old stage 82-year-old stage 66-year-old stage 19-year-old stage 79-year-old stage 70-year-old stage 77-year-old stage 21-year-old stage 32-year-old stage 50-year-old stage 86-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 93-year-old stage 52-year-old stage 60-year-old stage 22-year-old stage 44-year-old stage 48-year-old stage 64-year-old stage 89-year-old stage 45-year-old stage 25-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 67-year-old stage 40-year-old stage 75-year-old stage 27-year-old stage 95-year-old stage 23-year-old stage 37-year-old stage 71-year-old stage 78-year-old stage 28-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 20-year-old stage 47-year-old stage 31-year-old stage 56-year-old stage 96-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 34-year-old stage 51-year-old stage 49-year-old stage 42-year-old stage 35-year-old stage 53-year-old stage 94-year-old stage 72-year-old stage 73-year-old stage 30-year-old stage 24-year-old stage 26-year-old stage 88-year-old stage 84-year-old stage 39-year-old stage 97-year-old stage 90-year-old stage 69-year-old stage 43-year-old stage 29-year-old stage 38-year-old stage 87-year-old stage 41-year-old stage 81-year-old stage 55-year-old stage 83-year-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Common myeloid progenitor Be cell Cd1c-positive myeloid dendritic cell Mature gamma-delta t cell Naive b cell Innate lymphoid cell Cd4-positive, alpha-beta memory t cell Platelet Cd8-positive, alpha-beta regulatory t cell Naive regulatory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd4-positive, alpha-beta t cell Cd141-positive myeloid dendritic cell Transitional stage b cell Axl+ dendritic cell, human Cd16-negative, cd56-bright natural killer cell, human Common lymphoid progenitor Double negative thymocyte Cd14-positive, cd16-negative classical monocyte Cd8-positive, alpha-beta memory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell Intermediate monocyte Plasma cell Erythrocyte T cell Memory b cell Plasmacytoid dendritic cell Cd14-low, cd16-positive monocyte Memory regulatory t cell Memory ccr4-positive regulatory t cell Natural killer cell Basophil mast progenitor cell Cd8aa(i) thymocyte Cd16-positive, cd56-dim natural killer cell, human

Blood

27-year-old stage 37-year-old stage 28-year-old stage 36-year-old stage 31-year-old stage 34-year-old stage 35-year-old stage 30-year-old stage 26-year-old stage 29-year-old stage

Cytomegalovirus infection

Female

10x 3' v3

Cell

Danio rerio

Pancreatic endocrine cell Neuromast supporting cell Taste receptor cell Osteoblast Fast muscle myoblast Myeloid lineage restricted progenitor cell Slow muscle cell Muscle precursor cell Primordial germ cell Schwann cell Glutamatergic neuron Notochordal sheath cell Slow muscle myoblast Endodermal cell Hatching gland cell Cardiac endothelial cell Epidermal mucus secreting cell Hepatoblast Xanthoblast Peridermal cell Cardiac muscle cell Glial cell Nonkeratinized cell of epidermis Eye photoreceptor cell Ectodermal cell Muscle cell Gabaergic neuron Ionocyte Macrophage Cranial motor neuron Photoreceptor cell Kolmer-agduhr neuron Neural crest cell Mesothelial cell Rohon-beard neuron Neuroblast (sensu vertebrata) Glycinergic neuron Stromal cell Mesodermal cell Epithelial cell Type b pancreatic cell Pancreatic d cell Neutrophil Adaxial cell Tendon cell Splanchnic mesodermal cell Xanthophore cell Sensory hair cell Olfactory epithelial cell Myoblast Endocrine cell Neural progenitor cell Notochordal vacuole cell Motor neuron Spinal cord interneuron Auditory epithelial supporting cell Hepatocyte Podocyte Migratory neural crest cell Multiciliated epithelial cell Retinal progenitor cell Pericyte Endothelial cell Mesenchymal cell Lens epithelial cell Lens fiber cell Pancreatic a cell Neurecto-epithelial cell Epidermal cell Migratory cranial neural crest cell Retinal bipolar neuron Odontoblast Connective tissue cell Neuroplacodal cell Erythrocyte Amacrine cell Enteric neuron Notochordal cell Thyroid follicular cell Enterocyte Kidney proximal convoluted tubule epithelial cell Hypertrophic chondrocyte Premigratory neural crest cell Retinal pigment epithelial cell Chondroblast Smooth muscle cell Retinal ganglion cell Neuron Lateral motor column neuron Epithelial cell of distal tubule Purkinje cell Epithelial cell of exocrine pancreas Retina horizontal cell Chondrocyte Melanocyte Epithelial cell of esophagus Iridophore Fast muscle cell Melanoblast Goblet cell

Semicircular canal Intestinal epithelium Skin epidermis Ganglionic layer of retina Interrenal primordium Inner nuclear layer of retina Zona limitans intrathalamica Pronephros Periderm Heart primordium Fast muscle tissue Head Blood Cranial ganglion Anterior segment of eyeball Olfactory system Hatching gland Hypochord Pronephric mesoderm Lens vesicle Lateral line ganglion Leptomeninx Parachordal cartilage Proepicardium Ventricular zone Pigmented layer of retina Diencephalon Liver Forebrain Posterior neural tube Proximal convoluted tubule Genitourinary system Distal tubule Cloaca Dorsolateral placode Cerebellum Periocular mesenchyme Habenula Blood island Enteric ganglion Telencephalon Esophagus Meninx Hypaxial musculature Liver primordium Lens of camera-type eye Presomitic mesoderm Lateral line Tooth placode Pancreas Midbrain-hindbrain boundary Pharyngeal arch Olfactory epithelium Notochord Gonad primordium Corpuscles of stannius Pancreatic epithelial bud Brain Neural rod Endocardium Multicellular anatomical structure Crista ampullaris Midbrain tegmentum Somite Anterior lateral plate mesoderm Integument Myotome Pharynx Slow muscle tissue Epibranchial ganglion Thyroid follicle Midbrain Cleithrum Trigeminal ganglion Pectoral appendage bud Pronephric glomerulus Pharyngeal pouch Adenohypophysis Sclerotome Neural crest Retina Macula Taste bud Pectoral fin Bulbus arteriosus Spinal cord Trunk neural crest Craniocervical muscle Intestine Lateral plate mesoderm Hindbrain Endocrine pancreas Median fin fold Swim bladder Vestibulocochlear ganglion Midbrain tectum Floor plate Outer nuclear layer of retina Head connective tissue Ear vesicle Ectoderm-derived structure Blood vessel endothelium Heart Lymphatic vessel endothelium

Pharyngula:high-pec Segmentation:26+ somites Pharyngula:prim-5 Hatching:pec-fin Hatching:long-pec Segmentation:20-25 somites Segmentation:14-19 somites Larval:protruding-mouth Pharyngula:prim-15 Pharyngula:prim-25

Normal

Sci-rna-seq3

Nucleus

Homo sapiens

Common myeloid progenitor Be cell Cd1c-positive myeloid dendritic cell Mature gamma-delta t cell Naive b cell Innate lymphoid cell Cd4-positive, alpha-beta memory t cell Platelet Cd8-positive, alpha-beta regulatory t cell Naive regulatory t cell Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Effector memory cd4-positive, alpha-beta t cell Cd141-positive myeloid dendritic cell Transitional stage b cell Axl+ dendritic cell, human Cd16-negative, cd56-bright natural killer cell, human Common lymphoid progenitor Double negative thymocyte Cd14-positive, cd16-negative classical monocyte Cd8-positive, alpha-beta memory t cell Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell Intermediate monocyte Plasma cell Erythrocyte T cell Memory b cell Plasmacytoid dendritic cell Cd14-low, cd16-positive monocyte Memory regulatory t cell Memory ccr4-positive regulatory t cell Natural killer cell Basophil mast progenitor cell Cd8aa(i) thymocyte Cd16-positive, cd56-dim natural killer cell, human

Blood

32-year-old stage 33-year-old stage 27-year-old stage 37-year-old stage 28-year-old stage 36-year-old stage 31-year-old stage 34-year-old stage 35-year-old stage 30-year-old stage 26-year-old stage 29-year-old stage

Cytomegalovirus infection

Male

10x 3' v3

Cell

Mus musculus

L4/5 intratelencephalic projecting glutamatergic neuron of the primary motor cortex L2/3-6 intratelencephalic projecting glutamatergic neuron Pyramidal neuron Glutamatergic neuron Astrocyte Oligodendrocyte L2/3 intratelencephalic projecting glutamatergic neuron of the primary motor cortex Vascular leptomeningeal cell (mmus) L6 intratelencephalic projecting glutamatergic neuron of the primary motor cortex Sncg gabaergic interneuron L6 corticothalamic-projecting glutamatergic cortical neuron L5/6 near-projecting glutamatergic neuron of the primary motor cortex Sst gabaergic interneuron Lamp5 gabaergic interneuron L6b glutamatergic cortical neuron Corticothalamic-projecting glutamatergic cortical neuron Sst chodl gabaergic interneuron Pericyte Endothelial cell Cajal-retzius cell Hippocampal neuron Central nervous system macrophage L5 extratelencephalic projecting glutamatergic cortical neuron Vip gabaergic interneuron Pvalb gabaergic interneuron Meis2 expressing cortical gabaergic cell Cortical interneuron

Subicular complex Entorhinal cortex Hippocampal formation Posterior parietal association areas Primary somatosensory cortex Medial orbital frontal cortex Temporal cortex Agranular insular cortex Anterior cingulate cortex Parietal cortex Primary motor cortex Primary visual cortex Frontal lobe Auditory cortex Retrosplenial granular cortex Lateral visual area Visual cortex

7-week-old stage 9-week-old stage 8-week-old stage

Normal

Female Male

10x 3' v2

Cell

Homo sapiens

Taste receptor cell Myofibroblast cell Myoepithelial cell Common myeloid progenitor Transit amplifying cell of small intestine Slow muscle cell Acinar cell Best4+ enterocyte Luminal cell of prostate epithelium Radial glial cell Schwann cell Paneth cell of colon Pancreatic ductal cell Pulmonary alveolar type 1 cell Capillary endothelial cell Enterochromaffin-like cell Activated cd8-positive, alpha-beta t cell Ciliated epithelial cell Cd8-positive, alpha-beta thymocyte Mural cell Mesenchymal stem cell Erythroid progenitor cell Cardiac endothelial cell Progenitor cell of mammary luminal epithelium Classical monocyte Innate lymphoid cell Corneal epithelial cell Male germ cell Small intestine goblet cell Endothelial cell of lymphatic vessel Glial cell Eye photoreceptor cell Cd4-positive, alpha-beta t cell Pulmonary alveolar type 2 cell Muscle cell Club cell Platelet Non-classical monocyte Endothelial cell of arteriole Enterocyte of epithelium proper of jejunum Ionocyte Leukocyte Macrophage Pancreatic acinar cell Naive thymus-derived cd4-positive, alpha-beta t cell Skeletal muscle satellite stem cell Mesothelial cell Enterocyte of epithelium proper of duodenum Medullary thymic epithelial cell Neuro-medullary thymic epithelial cell Epithelial fate stem cell Basal cell of prostate epithelium Enterocyte of epithelium of large intestine Stromal cell Glandular secretory epithelial cell Interstitial cell of cajal Enteroendocrine cell of small intestine Ventricular cardiac muscle cell Mast cell Spermatocyte Basal cell Stratified squamous epithelial cell Endothelial cell of vascular tree Tracheal goblet cell Cd4-positive, alpha-beta thymocyte Epithelial cell Type b pancreatic cell Multiciliated columnar cell of tracheobronchial tree Fibroblast of breast Duct epithelial cell Erythroid lineage cell Adipocyte Enterocyte of epithelium proper of small intestine Pancreatic d cell Thymic fibroblast type 2 Endothelial cell of venule Neutrophil Monocyte Tendon cell Myo-medullary thymic epithelial cell Myometrial cell Epithelial cell of uterus Myeloid dendritic cell Acinar cell of salivary gland Enteroendocrine cell Salivary gland cell Blood vessel smooth muscle cell Luminal epithelial cell of mammary gland Respiratory tract goblet cell Type l enteroendocrine cell Hematopoietic cell Keratocyte Stromal cell of ovary Lung multiciliated epithelial cell Tuft cell of colon Retinal blood vessel endothelial cell Intrahepatic cholangiocyte Vein endothelial cell Microglial cell Granulocyte Serous cell of epithelium of trachea Leydig cell Bladder urothelial cell Regular atrial cardiac myocyte Vascular associated smooth muscle cell Colon macrophage Fibroblast Transit amplifying cell of colon Naive thymus-derived cd8-positive, alpha-beta t cell Hepatocyte Intestinal tuft cell Vestibular dark cell Hematopoietic precursor cell Hematopoietic stem cell Enterocyte of epithelium proper of ileum Tissue-resident macrophage Pericyte Endothelial cell Myeloid leukocyte Ovarian surface epithelial cell Pancreatic stellate cell Pancreatic pp cell Adventitial cell Mesenchymal cell Sebocyte Paneth cell of epithelium of small intestine Pancreatic a cell Intermediate monocyte B cell Thymocyte Cd8-positive, alpha-beta t cell Oocyte Mueller cell Hepatic stellate cell Kidney epithelial cell Retinal bipolar neuron Bronchial smooth muscle cell Activated cd4-positive, alpha-beta t cell Connective tissue cell Enteroglial cell Plasma cell Erythrocyte Regulatory t cell Fibroblast of cardiac tissue Mature nk t cell T cell Colon endothelial cell Mesenchymal stem cell of adipose tissue Intestinal crypt stem cell of small intestine Plasmacytoid dendritic cell Theca cell Intestinal crypt stem cell of colon Spermatid Mucus secreting cell Thymic fibroblast type 1 Alveolar adventitial fibroblast Retinal pigment epithelial cell Peritubular myoid cell Conjunctival epithelial cell T follicular helper cell Smooth muscle cell Basophil Retinal ganglion cell Neuron Pulmonary ionocyte Spermatogonium Large intestine goblet cell Retina horizontal cell Natural killer cell Endothelial cell of artery Tongue muscle cell Gamma-delta t cell Melanocyte Langerhans cell Supporting cell of vestibular epithelium Fast muscle cell Myeloid cell Mononuclear phagocyte Serous cell of epithelium of bronchus

Posterior part of tongue Posterior segment of eyeball Submandibular gland Stomach smooth muscle Blood Anterior segment of eyeball Coronary artery Ileum Uterus Thymus Vasculature Mesenteric lymph node Cortex of kidney Liver Skin of abdomen Spleen Parotid gland Brown adipose tissue Testis Right ovary Bladder organ Sublingual gland Lung Inferior vena cava Small intestine Abdominal aorta Ovary Muscle of abdomen Trachea Mammary gland Kidney Muscle of pelvic diaphragm Mucosa of stomach Inguinal lymph node Endometrium Sclera Utricle of membranous labyrinth Jejunum Cardiac ventricle Aorta Muscularis mucosae of stomach Adipose tissue Tongue Crista ampullaris Subcutaneous adipose tissue Prostate gland Right cardiac atrium Cardiac atrium Skin of chest Retinal neural layer Conjunctiva Eyelid Eye Chorioretinal region Sigmoid colon Cornea Duodenum Lacrimal gland Myometrium Left coronary artery Bone marrow Thoracic aorta Endocrine pancreas Left ovary Ascending colon Stomach Exocrine pancreas Heart right ventricle Skin of body Buccal mucosa Ocular surface region Anterior part of tongue Heart Large intestine Lymph node

57-year-old stage 46-year-old stage 60-year-old stage 22-year-old stage 45-year-old stage 74-year-old stage 33-year-old stage 67-year-old stage 40-year-old stage 37-year-old stage 36-year-old stage 59-year-old stage 56-year-old stage 61-year-old stage 42-year-old stage 26-year-old stage 69-year-old stage 38-year-old stage 55-year-old stage

Normal

Female Male

Smart-seq2 10x 5' v2 10x 3' v3 Smart-seq3

Cell

Homo sapiens

Radial glial cell Mural cell Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Macrophage Mast cell Neutrophil Monocyte Microglial cell Malignant cell Endothelial cell B cell Dendritic cell Plasma cell Neuron Natural killer cell Mature t cell

Right temporal lobe Basal ganglion Forebrain Left temporal lobe Right parietal lobe Temporoparietal junction Right frontal lobe Occipital lobe Brain Left frontal lobe Parietal lobe Temporal lobe Right occipital lobe Frontal lobe Left parietal lobe

80-year-old stage 66-year-old stage 70-year-old stage 77-year-old stage 21-year-old stage 50-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 44-year-old stage 48-year-old stage 64-year-old stage 45-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 67-year-old stage 71-year-old stage Adult stage 78-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 34-year-old stage 51-year-old stage 42-year-old stage 35-year-old stage 53-year-old stage 72-year-old stage 24-year-old stage 69-year-old stage 55-year-old stage 83-year-old stage

Glioblastoma

Female Male

Smart-seq2 Cel-seq2 10x 5' v1 10x 3' v3 Bd rhapsody whole transcriptome analysis Drop-seq 10x 3' v2 Strt-seq Microwell-seq

Cell Nucleus

Macaca mulatta

Glutamatergic neuron

Cerebellar vermis Entorhinal cortex Brodmann (1909) area 19 Inferior temporal gyrus Hippocampal formation Cerebrocerebellum Lateral geniculate body Primary somatosensory cortex Perirhinal cortex Corpus callosum Anterior cingulate cortex Primary auditory cortex Dorsolateral prefrontal cortex Pons Primary motor cortex Superior temporal sulcus Midbrain Amygdala Primary visual cortex Superior parietal cortex Medulla oblongata Inferior parietal cortex Ventral lateral nucleus of thalamus Medial dorsal nucleus of thalamus Nucleus accumbens Head of caudate nucleus Prefrontal cortex

Prime adult stage Juvenile stage

Normal

Female Male

Sci-rna-seq3

Nucleus

Homo sapiens

Schwann cell Trabecular meshwork cell Lymphocyte Macrophage Myelinating schwann cell Non-pigmented ciliary epithelial cell Mast cell Ciliary muscle cell Fibroblast Pigmented ciliary epithelial cell Blood vessel endothelial cell Pericyte Endothelial cell B cell Melanocyte

Eye trabecular meshwork Ciliary body Uvea

85-year-old stage 82-year-old stage 66-year-old stage 16-year-old stage 70-year-old stage 18-year-old stage 77-year-old stage 21-year-old stage 50-year-old stage 57-year-old stage 76-year-old stage 62-year-old stage 46-year-old stage 52-year-old stage 60-year-old stage 80 year-old and over stage 44-year-old stage 64-year-old stage 65-year-old stage 67-year-old stage 27-year-old stage 71-year-old stage 78-year-old stage 63-year-old stage 54-year-old stage 20-year-old stage 47-year-old stage 56-year-old stage 58-year-old stage 68-year-old stage 61-year-old stage 15-year-old stage 34-year-old stage 51-year-old stage 53-year-old stage 17-year-old stage 72-year-old stage 73-year-old stage 30-year-old stage 24-year-old stage 69-year-old stage 43-year-old stage 81-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Myofibroblast cell Pro-b cell Myoepithelial cell Best4+ enterocyte Transit amplifying cell Capillary endothelial cell Peptic cell Colonocyte Naive b cell Igg plasma cell Type x enteroendocrine cell Afferent neuron Type ec enteroendocrine cell Cd4-positive, alpha-beta memory t cell Endothelial cell of lymphatic vessel Muscle cell Intestinal crypt stem cell Ionocyte Iga plasma cell Fibroblastic reticular cell Macrophage Naive thymus-derived cd4-positive, alpha-beta t cell Mesothelial cell Neuroblast (sensu vertebrata) Mucosal invariant t cell Interstitial cell of cajal Mast cell Basal cell Type g enteroendocrine cell Mesodermal cell Epithelial cell Cd16-negative, cd56-bright natural killer cell, human Paneth cell Glandular cell of esophagus Monocyte Interneuron Megakaryocyte Immature pericyte Acinar cell of salivary gland Enteroendocrine cell Progenitor cell Plasmablast Vein endothelial cell Dendritic cell, human Motor neuron Cd8-positive, alpha-beta memory t cell Vascular associated smooth muscle cell Conventional dendritic cell Fibroblast Naive thymus-derived cd8-positive, alpha-beta t cell Group 3 innate lymphoid cell Angiogenic pericyte Effector memory cd8-positive, alpha-beta t cell Parietal cell T-helper 17 cell Salivary gland glandular cell Pericyte Endothelial cell Cycling basal cell Follicular dendritic cell Tuft cell of small intestine Mesenchymal cell Mucous neck cell of gastric gland Trem2-positive macrophage Cycling t cell Plasmacytoid dendritic cell, human Enteroglial cell Erythrocyte Regulatory t cell Memory b cell Enteric neuron Precursor b cell Enterocyte Igm plasma cell Inhibitory motor neuron Foveolar cell of stomach T follicular helper cell Fibroblast of gingiva Smooth muscle cell Gastrointestinal tract (lamina propria) macrophage Eosinophil Keratinocyte Neutrophil progenitor cell Endothelial cell of artery Gamma-delta t cell Melanocyte Cd16-positive, cd56-dim natural killer cell, human Langerhans cell M cell of gut Germinal center b cell Intestine goblet cell

Epithelium of esophagus Gingiva Lamina propria of mucosa of colon Body of stomach Duodenal epithelium Ileum Rectum Ileum lamina propria Mesenteric lymph node Parotid gland Epithelium of rectum Caecum Small intestine Esophagus Colonic epithelium Epithelium of stomach Jejunum Transverse colon Labial gland Epithelium of small intestine Pyloric antrum Lamina propria of small intestine Sigmoid colon Periodontium Duodenum Ileal epithelium Pylorus Colon Intestine Descending colon Ascending colon Stomach Rectosigmoid junction Buccal mucosa Vermiform appendix Colonic mucosa

80-year-old stage 9th week post-fertilization stage 85-year-old stage 66-year-old stage 70-year-old stage Young adult stage Fourth decade stage 77-year-old stage 13th week post-fertilization stage 12th week post-fertilization stage 21-year-old stage 32-year-old stage 50-year-old stage 86-year-old stage 57-year-old stage 76-year-old stage 10th week post-fertilization stage 62-year-old stage Prime adult stage 46-year-old stage 52-year-old stage 60-year-old stage 22-year-old stage Carnegie stage 22 Juvenile stage (5-14 yo) 44-year-old stage 48-year-old stage 45-year-old stage 74-year-old stage 33-year-old stage 65-year-old stage 67-year-old stage Embryonic stage Eighth decade stage 75-year-old stage 23-year-old stage 37-year-old stage Adult stage 11th week post-fertilization stage 21st week post-fertilization stage 6-year-old stage 28-year-old stage 19th week post-fertilization stage 63-year-old stage 17th week post-fertilization stage 59-year-old stage 54-year-old stage Fifth decade stage 31-year-old stage 2-month-old stage 15th week post-fertilization stage 56-year-old stage 58-year-old stage Pediatric stage 68-year-old stage 61-year-old stage 34-year-old stage Carnegie stage 19 51-year-old stage 35-year-old stage 16th week post-fertilization stage 10-year-old stage 72-year-old stage 30-year-old stage 24-year-old stage 18th week post-fertilization stage Seventh decade stage 26-year-old stage 84-year-old stage 12-year-old stage 39-year-old stage Newborn stage (0-28 days) 4-year-old stage Carnegie stage 17 9-year-old stage Third decade stage 43-year-old stage 29-year-old stage 87-year-old stage 41-year-old stage Carnegie stage 18 81-year-old stage 55-year-old stage Carnegie stage 16 Sixth decade stage

Normal

Female Male

10x 5' v2 10x 5' v1 10x 3' v3 10x 5' transcription profiling 10x 3' v1 10x 3' v2

Cell

Mus musculus musculus

Astrocyte Oligodendrocyte precursor cell Glial cell Oligodendrocyte Macrophage Microglial cell Fibroblast Ependymal cell Brain microvascular endothelial cell Neuron

Normal

10x 3' v3