Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron Caudal ganglionic eminence derived interneuron Cerebral cortex endothelial cell Vascular leptomeningeal cell Oligodendrocyte precursor cell Oligodendrocyte Astrocyte of the cerebral cortex Sncg gabaergic interneuron Near-projecting glutamatergic cortical neuron Sst gabaergic interneuron Microglial cell Lamp5 gabaergic interneuron L6b glutamatergic cortical neuron Corticothalamic-projecting glutamatergic cortical neuron L5 extratelencephalic projecting glutamatergic cortical neuron Vip gabaergic interneuron Pvalb gabaergic interneuron Pvalb chandelier gabaergic interneuron

Dorsolateral prefrontal cortex

50-year-old stage 60-year-old stage 42-year-old stage 43-year-old stage 29-year-old stage

Normal

Female Male

10x 3' v3

Nucleus

Homo sapiens

Lymphocyte Macrophage Blood vessel smooth muscle cell Fibroblast Microcirculation associated smooth muscle cell Endothelial cell of artery

Right coronary artery Coronary artery Pulmonary artery Abdominal aorta Iliac artery Ascending aorta Bulb of aorta Carotid artery segment Arch of aorta Descending aorta

46-year-old stage 48-year-old stage 67-year-old stage 39-year-old stage 69-year-old stage 38-year-old stage

Normal

Female Male

10x 3' v3

Cell

Homo sapiens

Astrocyte Oligodendrocyte precursor cell Oligodendrocyte Microglial cell Endothelial cell Neuron

Frontal cortex

82-year-old stage 66-year-old stage 70-year-old stage 86-year-old stage 62-year-old stage 74-year-old stage 65-year-old stage 67-year-old stage 63-year-old stage 96-year-old stage 58-year-old stage 68-year-old stage 73-year-old stage 88-year-old stage 84-year-old stage 83-year-old stage

Normal Frontotemporal dementia

Female Male

10x 3' v3

Nucleus

Homo sapiens

Platelet Naive thymus-derived cd4-positive, alpha-beta t cell Effector cd8-positive, alpha-beta t cell Transitional stage b cell Effector memory cd4-positive, alpha-beta t cell, terminally differentiated Cd14-positive monocyte Cd8-positive, alpha-beta cytotoxic t cell Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Central memory cd8-positive, alpha-beta t cell B cell Cd8-positive, alpha-beta t cell Plasmacytoid dendritic cell, human Plasma cell T cell Cd14-low, cd16-positive monocyte Natural killer cell Gamma-delta t cell

Hiv infectious disease

10x 3' v3

Drosophila melanogaster

Adult olfactory receptor neuron gr21a/63a Lamina monopolar neuron l4 Transmedullary y neuron tmy5a Medulla intrinsic neuron mi15 Primary pigment cell Medulla intrinsic neuron mi1 Proximal medullary amacrine neuron pm4 Distal medullary amacrine neuron dm9 Columnar neuron t1 Transmedullary y neuron tmy4 Transmedullary neuron tm9 Inner photoreceptor cell Epidermal cell Distal medullary amacrine neuron dm10 Auditory sensory neuron Proximal medullary amacrine neuron pm2 Ensheathing glial cell Adult astrocyte-like glial cell Lobula columnar neuron lc17 Somatic muscle cell Gravity sensitive johnston organ neuron Lobula columnar neuron lc12 Kenyon cell Ocellar retinula cell Adult lamina epithelial glial cell Transmedullary neuron tm3 Centrifugal neuron c3 Adult brain cell body glial cell T2a neuron Adult optic chiasma glial cell T2 neuron Medulla intrinsic neuron mi9 Lamina wide-field 1 neuron Cone cell Distal medullary amacrine neuron dm11 Transmedullary neuron tm20 Olfactory receptor neuron Transmedullary y neuron tmy8 Photoreceptor cell of bolwig organ Lamina monopolar neuron l3 Pigment cell Lamina monopolar neuron l1 Lamina monopolar neuron l2 Transmedullary neuron tm2 Lamina monopolar neuron l5 Medulla intrinsic neuron mi4 Adult somatic muscle cell of head Distal medullary amacrine neuron dm3 Adult brain perineurial glial cell Transmedullary neuron tm5c Outer photoreceptor cell Optic-lobe-associated cortex glial cell Gamma kenyon cell Antennal lobe projection neuron Photoreceptor cell r7 Adult lamina marginal glial cell Alpha'/beta' kenyon cell Hemocyte Distal medullary amacrine neuron dm8a Dopaminergic neuron Centrifugal neuron c2 Photoreceptor cell r8 Adult dopaminergic pam neuron Lamina wide-field 2 neuron T3 neuron Transmedullary neuron tm1 T4 neuron Subperineurial glial cell Adult lobula astrocyte-like glial cell Lobula columnar neuron lc10 Distal medullary amacrine neuron dm12 Transmedullary neuron tm4 Alpha/beta kenyon cell T5 neuron Johnston organ neuron Transmedullary y neuron tmy14

Head

Day 5 of adulthood

Normal

Female Male Mixed sex

10x 3' v3

Homo sapiens

Contractile cell Lymphocyte Macrophage Myelinating schwann cell Adipocyte Perineuronal satellite cell Fibroblast Sensory neuron of dorsal root ganglion Endothelial cell

Seventh thoracic dorsal root ganglion Fourth lumbar dorsal root ganglion Fifth lumbar dorsal root ganglion

16-year-old stage 21-year-old stage 48-year-old stage 25-year-old stage Life cycle 31-year-old stage 38-year-old stage

Normal

Female Male

10x genomics v3 10x genomics v2

Homo sapiens

Oligodendrocyte

Middle temporal gyrus

80-year-old stage 85-year-old stage 82-year-old stage 70-year-old stage 77-year-old stage 50-year-old stage 86-year-old stage 60-year-old stage 80 year-old and over stage 89-year-old stage 65-year-old stage 75-year-old stage Adult stage 78-year-old stage 68-year-old stage 72-year-old stage 88-year-old stage 84-year-old stage 69-year-old stage 43-year-old stage 29-year-old stage 87-year-old stage 81-year-old stage 83-year-old stage

Normal Dementia

Female Male

10x 3' v3 10x multiome

Nucleus

Drosophila melanogaster

Eo sensory organ accessory cell Multidendritic neuron Muscle cell Gustatory receptor neuron Adult tracheocyte Cholinergic neuron Astrocyte-like glial cell Leg muscle motor neuron Female germline cell Neurosecretory neuron Adult astrocyte-like glial cell Escort cell Somatic muscle cell Enteroendocrine cell Spermatocyte Adult glial cell Germline cell Leg taste bristle chemosensory neuron Cns surface glial cell Tracheolar cell Follicle cell Somatic precursor cell Epithelial cell Chordotonal neuron Neuron Sensory neuron Cardial cell Perineurial glial cell Fat cell Indirect flight muscle cell Hemocyte Cell body glial cell Male germline cell Subperineurial glial cell Oenocyte Neuron Malpighian tubule type i cell Adult oenocyte Polar follicle cell Glial cell

Day 5 of adulthood

Normal

Female Male Mixed sex

10x 3' v3

Mus musculus

Bladder cell Circulating fibrocyte Smooth muscle cell of the pulmonary artery Kupffer cell Promonocyte Pulmonary interstitial fibroblast Pancreatic ductal cell Pulmonary alveolar type 1 cell Epithelial cell of thymus Naive b cell Endothelial cell of hepatic sinusoid Mesenchymal stem cell Classical monocyte Astrocyte Oligodendrocyte precursor cell Endothelial cell of lymphatic vessel Endothelial cell of coronary artery Kidney loop of henle ascending limb epithelial cell Oligodendrocyte Tuft cell of large intestine Cd4-positive, alpha-beta t cell Pulmonary alveolar type 2 cell Club cell Pulmonary neuroendocrine cell Neural stem cell Intestinal crypt stem cell Non-classical monocyte Leukocyte Lymphocyte Macrophage Medium spiny neuron Pancreatic acinar cell Granulocyte monocyte progenitor cell Endocardial cell Enterocyte of epithelium of large intestine Late pro-b cell Stromal cell Kidney interstitial fibroblast Dn4 thymocyte Kidney collecting duct principal cell Basal cell Epithelial cell Type b pancreatic cell Multiciliated columnar cell of tracheobronchial tree Megakaryocyte-erythroid progenitor cell Pancreatic d cell Bergmann glial cell Neutrophil Basal epithelial cell of tracheobronchial tree Monocyte Interneuron Myeloid dendritic cell Enteroendocrine cell Basal cell of epidermis Granulocytopoietic cell Keratinocyte stem cell Luminal epithelial cell of mammary gland Immature b cell Lymphoid lineage restricted progenitor cell Epithelial cell of proximal tubule Vein endothelial cell Microglial cell Granulocyte Neuroepithelial stem cell Bladder urothelial cell Regular atrial cardiac myocyte Fibroblast Mature alpha-beta t cell Brain pericyte Hepatocyte Hematopoietic stem cell Ependymal cell Pericyte Endothelial cell Skeletal muscle satellite cell Myeloid leukocyte Regular ventricular cardiac myocyte Pancreatic stellate cell Pancreatic pp cell Adventitial cell Pancreatic a cell Intermediate monocyte B cell Thymocyte Cardiac valve cell Epidermal cell Cd8-positive, alpha-beta t cell Lung macrophage Bronchial smooth muscle cell Dendritic cell Plasma cell Mesangial cell Regulatory t cell Fibroblast of cardiac tissue Mature nk t cell T cell Mesenchymal stem cell of adipose tissue Plasmacytoid dendritic cell Smooth muscle cell of trachea Secretory cell Precursor b cell Mucus secreting cell Fenestrated endothelial cell Kidney collecting duct epithelial cell Fibroblast of lung Early pro-b cell Smooth muscle cell Basophil Proerythroblast Respiratory basal cell Neuron Large intestine goblet cell Epithelial cell of large intestine Keratinocyte Natural killer cell Chondrocyte Professional antigen presenting cell Aortic endothelial cell Myeloid cell

Thymus Liver Spleen Brown adipose tissue Lung Gonadal fat pad Mesenteric fat pad Trachea Mammary gland Kidney Pancreas Aorta Brain Tongue Subcutaneous adipose tissue Diaphragm Limb muscle Bone marrow Skin of body Bladder lumen Heart Large intestine

3-month-old stage 20-month-old stage and over 18-month-old stage

Normal

Female Male

Smart-seq2

Cell

Homo sapiens

L2/3-6 intratelencephalic projecting glutamatergic neuron Caudal ganglionic eminence derived interneuron Cerebral cortex endothelial cell Vascular leptomeningeal cell Oligodendrocyte precursor cell Oligodendrocyte Astrocyte of the cerebral cortex Sncg gabaergic interneuron Near-projecting glutamatergic cortical neuron Sst gabaergic interneuron Microglial cell Lamp5 gabaergic interneuron L6b glutamatergic cortical neuron Corticothalamic-projecting glutamatergic cortical neuron L5 extratelencephalic projecting glutamatergic cortical neuron Vip gabaergic interneuron Pvalb gabaergic interneuron Pvalb chandelier gabaergic interneuron

Angular gyrus

42-year-old stage 29-year-old stage Sixth decade stage

Normal

Male

10x 3' v3

Nucleus

Homo sapiens

Neuroblast (sensu nematoda and protostomia)

Upper layers of the cortex Dorsal plus ventral thalamus Head Entorhinal cortex Cerebral subcortex Caudate-putamen Hippocampal formation Hypothalamus Diencephalon Forebrain Cerebellum Telencephalon Choroid plexus epithelium Cerebellar peduncular complex Frontal cortex Brain Striatum Pons Midbrain Lower layers of the cortex Medulla oblongata Hindbrain Cortex Midbrain tectum Basal forebrain Occipital cortex Metencephalon

9th week post-fertilization stage 13th week post-fertilization stage 12th week post-fertilization stage 10th week post-fertilization stage Carnegie stage 22 11th week post-fertilization stage 15th week post-fertilization stage 14th week post-fertilization stage Carnegie stage 15 Carnegie stage 20 Carnegie stage 18 Carnegie stage 16

Normal

10x 3' v3 10x 3' v2

Cell

Homo sapiens

Activated cd4-positive, alpha-beta t cell, human Lymphocyte Naive thymus-derived cd4-positive, alpha-beta t cell Mucosal invariant t cell Cd16-negative, cd56-bright natural killer cell, human Double negative thymocyte Naive thymus-derived cd8-positive, alpha-beta t cell Central memory cd4-positive, alpha-beta t cell Effector memory cd8-positive, alpha-beta t cell Cd4-positive, alpha-beta cytotoxic t cell Regulatory t cell Activated cd8-positive, alpha-beta t cell, human Natural killer cell Gamma-delta t cell

Blood

82-year-old stage 70-year-old stage Fourth decade stage 62-year-old stage 46-year-old stage 60-year-old stage 80 year-old and over stage 48-year-old stage 67-year-old stage Eighth decade stage 75-year-old stage 27-year-old stage 37-year-old stage 78-year-old stage 36-year-old stage 63-year-old stage 59-year-old stage 54-year-old stage Fifth decade stage 47-year-old stage 68-year-old stage 61-year-old stage 49-year-old stage 53-year-old stage 73-year-old stage Seventh decade stage 84-year-old stage 39-year-old stage Third decade stage 29-year-old stage 38-year-old stage Sixth decade stage

Normal Covid-19

Female Male

10x 3' v3 Seq-well 10x 3' v2

Cell